CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free

Images

 
Western Blot: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40.
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free [NBP2-70378] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI8B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 21-166 of human CD40(NP_001241) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD40
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:150
Theoretical MW
28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for CD40/TNFRSF5 Antibody (OTI8B8) - Azide and BSA Free

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD40/TNFRSF5 Antibody (NBP2-70378). (Showing 1 - 2 of 2 FAQ).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70378AF350
Alexa Fluor 405 NBP2-70378AF405
Alexa Fluor 488 NBP2-70378AF488
Alexa Fluor 532 NBP2-70378AF532
Alexa Fluor 594 NBP2-70378AF594
Alexa Fluor 647 NBP2-70378AF647
Alexa Fluor 700 NBP2-70378AF700
Alexa Fluor 750 NBP2-70378AF750
Biotin NBP2-70378B
DyLight 350 NBP2-70378UV
DyLight 405 NBP2-70378V
DyLight 488 NBP2-70378G
DyLight 550 NBP2-70378R
DyLight 594 NBP2-70378DL594
DyLight 650 NBP2-70378C
DyLight 680 NBP2-70378FR
DyLight 755 NBP2-70378IR
FITC NBP2-70378F
HRP NBP2-70378H
Janelia Fluor 549 NBP2-70378JF549
Janelia Fluor 646 NBP2-70378JF646

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Bioinformatics

Gene Symbol CD40