CD40/TNFRSF5 Antibody (G28-5)


Immunocytochemistry/ Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma .

Product Details

Reactivity HuSpecies Glossary
Applications Flow, Func, ICC/IF, IHC
0.5 mg/ml

Order Details

CD40/TNFRSF5 Antibody (G28-5) Summary

Additional Information
Clone G28-5 was used by HLDA to establish CD designation.
CD40 was used as immunogen.
IgG1 Kappa
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
Read Publications using DDX0130P-100.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris-NaCl (pH 8.0)
No Preservative
0.5 mg/ml
Ion exchange chromatography


This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for CD40/TNFRSF5 Antibody (G28-5)

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5


CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD40/TNFRSF5 Antibody (DDX0130P-100)(9)

Showing Publications 1 - 9 of 9.
Publications using DDX0130P-100 Applications Species
Wu HH, Ralph KL, Sepuldeva E Et al. An optimally designed anti-human CD40 antibody with potent B cell suppression for the treatment of autoimmune diseases International journal of pharmaceutics 2021-10-06 [PMID: 34624444]
Clark EA. A Short History of the B-Cell-Associated Surface Molecule CD40. Front Immunol 2014-09-01 [PMID: 25324844]
Clark EA et al. Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc. Natl. Acad. Sci. 1986. 83: 4494-4498. J Immunol. 2012-05-01 [PMID: 22517865]
Bishop GA. The power of monoclonal antibodies as agents of discovery: CD40 Revealed as a B lymphocyte costimulator. J Immunol 2012-05-01 [PMID: 22517864]
Lefevre EA et al. Fibrinogen is localized on dark zone follicular dendritic cells in vivo and enhances the proliferation and survival of a centroblastic cell line in vitro. J Leukoc Biol 2007-09-01 [PMID: 17550975]
Dahlenborg K et al. Signals sustaining human immunoglobulin V gene hypermutation in isolated germinal centre B cells. Immunology 2000-10-01 [PMID: 11012774]
Altenburg A et al. CD40 ligand-CD40 interaction induces chemokines in cervical carcinoma cells in synergism with IFN-gamma. J Immunol 1999-04-01 [PMID: 10201939]
de Saint Vis B et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity 1998-09-01 [PMID: 9768752]
Clark EA et al. Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc Natl Acad Sci U S A 1986-06-01 [PMID: 3487090]

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Product General Protocols

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ICC/IF Video Protocol

FAQs for CD40/TNFRSF5 Antibody (DDX0130P-100). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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Gene Symbol CD40