CD40 Ligand/TNFSF5

Immunocytochemistry: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - PBMC culturedin the presence of DDX-S2

Flow Cytometry: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - Surface cytometry analysis of CD40L expression

Immunofluorescence: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - IF stainingof L6 cells

In vitro assay: CD40 Ligand/TNFSF5 Lysate [DDX-S2]

CD40 Ligand/TNFSF5 Lysate [DDX-S2] - Freeze-dried L6 cells are part of the Blood B Booster technology intended to immortalizehuman B cells and to activate animal B cells

• Applications: Flow, Func, ICC/IF
• Host: Horse

CD40 Ligand/TNFSF5 Summary

• Preparation Method: Resuspended L6 cells can be kept at 4C under sterilized conditions for up to 2 months.
• Host: Horse
• Gene: CD40LG

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • In vitro assay
• Application Notes: Murine L cells were stably transfected with human CD40L (L6) and freeze-dried through proprietary process. These cells are primarily intended to activate human cells through triggering of CD40 surface antigen but were also demonstrated to activate B cells from several animal species. L6 cells successfully support the differentiation of human monocyte towards the dendritic cell lineage. L6 cells are included in the Human Blood B booster and the Animal Blood B Booster kits, and support B cell growth and Ig secretion, but can also used alone for transient cultures.

Reactivity Notes

Rat, Sheep, Dog, Cat, Pig, Horse, Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Store at -20C. Avoid freeze-thaw cycles. After opening store under sterile conditions.
• Preservative: No Preservative

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for CD40 Ligand/TNFSF5

• CD154 antigen
• CD154
• CD40 antigen ligand
• CD40 Ligand
• CD40L
• CD40-L
• CD40LG
• CD40LIGM
• gp39
• hCD40L
• HIGM1
• T-B cell-activating molecule
• T-BAM
• T-cell antigen Gp39
• TNF-related activation protein
• TNFSF5
• TNFSF5IMD3
• TRAP
• TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• tumor necrosis factor (ligand) superfamily member 5
• Tumor necrosis factor ligand superfamily member 5

Background

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

FAQs for CD40 Ligand/TNFSF5 (DDX-S2). (Showing 1 - 1 of 1 FAQ).

1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
   • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD40LG