Recombinant Mouse CD40 Ligand/TNFSF5 Protein

Bioactivity: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - CD40 Ligand/TNFSF5 induced IL-8 production by Human PBMCs.

SDS-Page: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - CD40 Ligand/TNFSF5 induced activity of HEK-blue (TM) CD40L cells.

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Concentration: LYOPH

Recombinant Mouse CD40 Ligand/TNFSF5 Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to Mouse CD40 Ligand/TNFSF5
  
  

  Source: E. coli

  Amino Acid Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

• Specificity: CD40 Ligand/TNFSF5 is a monomer, 16.4 kDa (149 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: HEK-blue((TM)) CD40L Activity, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CD40LG
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 16.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM arginine, pH7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 Protein

• CD154 antigen
• CD154
• CD40 antigen ligand
• CD40 Ligand
• CD40L
• CD40-L
• CD40LG
• CD40LIGM
• gp39
• hCD40L
• HIGM1
• T-B cell-activating molecule
• T-BAM
• T-cell antigen Gp39
• TNF-related activation protein
• TNFSF5
• TNFSF5IMD3
• TRAP
• TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• tumor necrosis factor (ligand) superfamily member 5
• Tumor necrosis factor ligand superfamily member 5

Background

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

FAQs for CD40 Ligand/TNFSF5 Recombinant Protein (NBP2-76185). (Showing 1 - 1 of 1 FAQ).

1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
   • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD40LG