Recombinant Mouse CD40 Ligand/TNFSF5 Protein

Images

 
Bioactivity: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - CD40 Ligand/TNFSF5 induced IL-8 production by Human PBMCs.
SDS-Page: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Mouse CD40 Ligand/TNFSF5 Protein [NBP2-76185] - CD40 Ligand/TNFSF5 induced activity of HEK-blue (TM) CD40L cells.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
LYOPH

Order Details

Recombinant Mouse CD40 Ligand/TNFSF5 Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Mouse CD40 Ligand/TNFSF5

Source: E. coli

Amino Acid Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Specificity
CD40 Ligand/TNFSF5 is a monomer, 16.4 kDa (149 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
HEK-blue((TM)) CD40L Activity, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CD40LG
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Theoretical MW
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM arginine, pH7.5
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 Protein

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for CD40 Ligand/TNFSF5 Recombinant Protein (NBP2-76185). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Bioinformatics

Gene Symbol CD40LG