Recombinant Mouse CD40 Ligand/TNFSF5 Protein Summary
An un-tagged bioactive recombinant protein corresponding to Mouse CD40 Ligand/TNFSF5
Source: E. coli
Amino Acid Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
CD40 Ligand/TNFSF5 is a monomer, 16.4 kDa (149 aa)
Protein is derived from genetically modified E. coli.
|Details of Functionality
HEK-blue((TM)) CD40L Activity, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).
| Protein/Peptide Type
>95%, by SDS-PAGE
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM arginine, pH7.5
>95%, by SDS-PAGE
Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 Protein
- CD154 antigen
- CD40 antigen ligand
- CD40 Ligand
- T-B cell-activating molecule
- T-cell antigen Gp39
- TNF-related activation protein
- TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
- tumor necrosis factor (ligand) superfamily member 5
- Tumor necrosis factor ligand superfamily member 5
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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