Recombinant Mouse CD40 Ligand/TNFSF5 Protein Summary
Description |
An un-tagged bioactive recombinant protein corresponding to Mouse CD40 Ligand/TNFSF5 Source: E. coli Amino Acid Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL |
Specificity |
CD40 Ligand/TNFSF5 is a monomer, 16.4 kDa (149 aa) |
Preparation Method |
Protein is derived from genetically modified E. coli. |
Details of Functionality |
HEK-blue((TM)) CD40L Activity, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL). |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
CD40LG |
Purity |
>95%, by SDS-PAGE |
Endotoxin Note |
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method). |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
16.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM arginine, pH7.5 |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
>95%, by SDS-PAGE |
Reconstitution Instructions |
Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. |
Notes
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 Protein
Background
CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is
guaranteed for 1 year from date of receipt.
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