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CD38 Antibody (1G7F4)

 (2 Publications)
 (1 Review)
 
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Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of A) Human Jurkat, B) Human Ramos and C) Mouse EL4 lysate using this antibody at 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: CD38 Antibody (1G7F4) [NBP2-25250] - HuH-7 cells (human liver cancer cell line) stained with Alexa Fluor 647 conjugated CD38 antibody (Catalog # NBP2-25265AF647). Image from ...read more
Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: CD38 Antibody (1G7F4) [NBP2-25250] - Mouse liver stained with this antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note membrane staining of Kupffer cells (macrophages of the ...read more
Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody (Catalog # NBP2-25265H). Image from verified customer review.
Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
1G7F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25250AF350
Alexa Fluor 405
NBP2-25265AF405
Alexa Fluor 488
NBP2-25265AF488
Alexa Fluor 532
NBP2-25250AF532
Alexa Fluor 594
NBP2-25250AF594
Alexa Fluor 647
NBP2-25265AF647
Alexa Fluor 700
NBP2-25265AF700
Alexa Fluor 750
NBP2-25250AF750
Biotin
NBP2-25265B
DyLight 350
NBP2-25265UV
DyLight 405
NBP2-25265V
DyLight 488
NBP2-25265G
DyLight 550
NBP2-25265R
DyLight 594
NBP2-25250DL594
DyLight 650
NBP2-25265C
DyLight 680
NBP2-25265FR
DyLight 755
NBP2-25265IR
FITC
NBP2-25265F
HRP
NBP2-25265H
Janelia Fluor 549
NBP2-25250JF549
Janelia Fluor 646
NBP2-25250JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide Free
NBP2-25265
Azide Free
NBP2-25265SS
100% Guarantee on every product

CD38 Antibody (1G7F4) Summary

Immunogen
Amino acids 71-197 of mouse CD38 were used as the immunogen for the antibody.
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
CD38
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 3-5 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control
CD38 Lysate (NBP2-66357)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25250 in the following applications:
Publications
Read Publications using
NBP2-25250 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD38 Antibody (1G7F4)

  • ADP-ribosyl cyclase 1
  • ADP-ribosyl Cyclase
  • ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
  • cADPr hydrolase 1
  • CD38 antigen (p45)
  • CD38 antigen
  • CD38 molecule
  • CD38
  • Cyclic ADP-ribose hydrolase 1
  • Cyclic ADP-ribose Hydrolase
  • EC 3.2.2.5
  • NAD(+) nucleosidase
  • T10

Background

CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 also functions in cell adhesion, signal transduction and calcium signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.
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NBP1-49045
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Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
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Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody clone 30-F11 at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. Image submitted via a verified customer review.
Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).
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CD38 Antibody
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CD38 Antibody (1G7F4)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
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Publications for CD38 Antibody (NBP2-25250)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Review for CD38 Antibody (NBP2-25250) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-25250:
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IHC-Fr
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD38 NBP2-25250
Enlarge
reviewed by:
Anonymous
 IHC-Fr Human 08/27/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedB cell lymphoma
SpeciesHuman
Lot061145577F401

Comments

CommentsDilution ratio: 1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X
Blocking details: 10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD38 Antibody (NBP2-25250). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best antibody anti-human CD38 for IHC-P?
    • We do have a number of CD38 antibodies validated in IHC-P, please use the filters on the left side of the search page to help find the product that mostly suits to your experimental design.

Control Lysate(s)

CD38 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP2-25265B
DyLight 350 Labeled NBP2-25265UV
DyLight 405 Labeled NBP2-25265V
DyLight 488 Labeled NBP2-25265G
DyLight 550 Labeled NBP2-25265R
DyLight 650 Labeled NBP2-25265C
DyLight 680 Labeled NBP2-25265FR
DyLight 755 Labeled NBP2-25265IR
HRP Labeled NBP2-25265H
Learn about Custom Antibody Labeling

Additional Array Products

Bioinformatics Tool for CD38 Antibody (NBP2-25250)

Discover related pathways, diseases and genes to CD38 Antibody (NBP2-25250). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD38 Antibody (NBP2-25250)

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Pathways for CD38 Antibody (NBP2-25250)

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PTMs for CD38 Antibody (NBP2-25250)

Learn more about PTMs related to CD38 Antibody (NBP2-25250).
 

Research Areas for CD38 Antibody (NBP2-25250)

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Blogs on CD38.

Check out the latest blog posts on CD38.
Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.
Multifunctional CD38
CD38 is a 42 kD type II transmembrane glycoprotein that uses nicotinamide adenine dinucleotide (NAD) as a substrate to form cyclic adenosine diphosphate ribose (cADPR). This novel multifunctional ectoenzyme has both cyclase and hydrolase enzymatic act...  Read full blog post.

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Recent Reviews

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Immunohistochemistry-Frozen CD38 NBP2-25250
Enlarge
 Anonymous
08/27/2017
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol CD38
Uniprot