![Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Simple-Western-NBP2-25250-img0005.jpg "Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P |
| Clone | 1G7F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Amino acids 71-197 of mouse CD38 were used as the immunogen for the antibody. |
| Predicted Species | Rat (91%). Backed by our 100% Guarantee. |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD38 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Anonymous |
IHC-Fr | Human | 08/27/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
| Biotin Labeled | NBP2-25265B | |
| DyLight 350 Labeled | NBP2-25265UV | |
| DyLight 405 Labeled | NBP2-25265V | |
| DyLight 488 Labeled | NBP2-25265G | |
| DyLight 550 Labeled | NBP2-25265R | |
| DyLight 650 Labeled | NBP2-25265C | |
| DyLight 680 Labeled | NBP2-25265FR | |
| DyLight 755 Labeled | NBP2-25265IR | |
| HRP Labeled | NBP2-25265H |
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Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post. |
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Multifunctional CD38 CD38 is a 42 kD type II transmembrane glycoprotein that uses nicotinamide adenine dinucleotide (NAD) as a substrate to form cyclic adenosine diphosphate ribose (cADPR). This novel multifunctional ectoenzyme has both cyclase and hydrolase enzymatic act... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of A) Human Jurkat, B) Human Ramos and C) Mouse EL4 lysate using this antibody at 4 ug/ml.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Western-Blot-NBP2-25250-img0003.jpg "Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of A) Human Jurkat, B) Human Ramos and C) Mouse EL4 lysate using this antibody at 4 ug/ml.")
![Immunocytochemistry/Immunofluorescence: CD38 Antibody (1G7F4) [NBP2-25250] - HuH-7 cells (human liver cancer cell line) stained with Alexa Fluor 647 conjugated CD38 antibody (Catalog # NBP2-25265AF647). Image from verified customer review.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Immunocytochemistry-Immunofluorescence-NBP2-25250-img0007.jpg "Immunocytochemistry/Immunofluorescence: CD38 Antibody (1G7F4) [NBP2-25250] - HuH-7 cells (human liver cancer cell line) stained with Alexa Fluor 647 conjugated CD38 antibody (Catalog # NBP2-25265AF647). Image from verified customer review.")
![Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Immunohistochemistry-Frozen-NBP2-25250-img0012.jpg "Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. This image was submitted via customer Review.")
![Immunohistochemistry-Paraffin: CD38 Antibody (1G7F4) [NBP2-25250] - Mouse liver stained with this antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note membrane staining of Kupffer cells (macrophages of the liver).](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Immunohistochemistry-Paraffin-NBP2-25250-img0004.jpg "Immunohistochemistry-Paraffin: CD38 Antibody (1G7F4) [NBP2-25250] - Mouse liver stained with this antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note membrane staining of Kupffer cells (macrophages of the liver).")
![Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody (Catalog # NBP2-25265H). Image from verified customer review.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Immunohistochemistry-Frozen-NBP2-25250-img0008.jpg "Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody (Catalog # NBP2-25265H). Image from verified customer review.")
![Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/CD38-Antibody-(1G7F4)-Simple-Western-NBP2-25250-img0011.jpg "Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Western-Blot-NBP1-84550-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0002.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/fullsize2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize2/CD34 Antibody (MEC 14.7)-Flow Cytometry-NB600-1071-img0002.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/fullsize2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunohistochemistry-Frozen-NBP1-19371-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize2/CD19-Antibody-(CB19)-Immunocytochemistry-Immunofluorescence-NBP2-25196-img0002.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize2/CD19-Antibody-(CB19)-Flow-Cytometry-NBP2-25196-img0013.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.](http://images.novusbio.com/fullsize2/CD19-Antibody-(CB19)-Flow-Cytometry-NBP2-25196-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunocytochemistry-NBP1-49045-img0011.jpg)
![Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.](http://images.novusbio.com/fullsize2/CD45-Antibody-(30-F11)-Immunohistochemistry-Paraffin-NB100-77417-img0006.jpg)
![Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody clone 30-F11 at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. Image submitted via a verified customer review.](http://images.novusbio.com/fullsize2/CD45-Antibody-(30-F11)-Immunohistochemistry-Frozen-NB100-77417-img0008.jpg)
![Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).](http://images.novusbio.com/fullsize2/CD45-Antibody-(30-F11)-Flow-Cytometry-NB100-77417-img0004.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Western Blot: ZAP70 Antibody (3D2A4) [NB110-60490] - Analysis using ZAP70 mouse mAb against Jurkat cell lysate (1).](http://images.novusbio.com/fullsize2/ZAP70-Antibody-(3D2A4)-Western-Blot-NB110-60490-img0007.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody (3D2A4) [NB110-60490] - Analysis of human lymph tumor, showing cytoplasmic localization using ZAP70 mouse mAb with DAB staining](http://images.novusbio.com/fullsize2/ZAP70-Antibody-(3D2A4)-Immunohistochemistry-Paraffin-NB110-60490-img0006.jpg)
![Western Blot: Cytokeratin 20 Antibody [NBP1-85599] - Analysis in human small intestine tissue.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-Western-Blot-NBP1-85599-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85599] - Analysis in human small intestine and liver tissues. Corresponding KRT20 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-Immunohistochemistry-Paraffin-NBP1-85599-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85599] - Staining of human liver shows no positivity in hepatocytes as expected](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-Immunohistochemistry-Paraffin-NBP1-85599-img0007.jpg)
![Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/ISG20 Antibody (1B2-3C9)-Western Blot-H00003669-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/ISG20-Antibody-(1B2-3C9)-Immunocytochemistry-Immunofluorescence-H00003669-M01-img0006.jpg)
![Sandwich ELISA: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/ISG20-Antibody-(1B2-3C9)-Sandwich-ELISA-H00003669-M01-img0007.jpg)
![Western Blot: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/Siglec-3-CD33-Antibody-(44M12D3)-Western-Blot-NBP2-22377-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize2/Siglec-3-CD33-Antibody-(44M12D3)-Immunohistochemistry-Paraffin-NBP2-22377-img0006.jpg)
![Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [HRP] [NBP2-25265H] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD38-Antibody-(1G7F4)-[HRP]-Immunohistochemistry-Frozen-NBP2-25265H-img0002.jpg "Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [HRP] [NBP2-25265H] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody. Image from verified customer review.")