CD38 Antibody (1G7F4)

Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. IHC-Fr image submitted by a verified customer review.

Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of (A) Human Jurkat, (B) Human Ramos and (C) Mouse EL4 lysate using this antibody at 4 ug/mL.

Immunocytochemistry/Immunofluorescence: CD38 Antibody (1G7F4) [NBP2-25250] - HuH-7 cells (human liver cancer cell line) stained with Alexa Fluor 647 conjugated CD38 antibody. Image using the Alexa Fluor 647 format of this antibody. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CD38 Antibody (1G7F4) [NBP2-25250] - Mouse liver stained with this antibody (5 ug/mL), peroxidase-conjugate and DAB chromogen. Note membrane staining of Kupffer cells (macrophages of the liver).

Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - Human lung cancer frozen tissue stained with HRP conjugated CD38 antibody (Catalog # NBP2-25265H). IHC-Fr image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 1G7F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD38 Antibody (1G7F4) Summary

• Immunogen: This CD38 Antibody (1G7F4) was developed against amino acids 71-197 of mouse CD38.
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD38
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Simple Western 1:50
  • Western Blot 3 - 5 ug/mL
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD38 Antibody (1G7F4)

• 2'-phospho-ADP-ribosyl cyclase
• 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20)
• 2'-phospho-cyclic-ADP-ribose transferase
• ADPRC 1
• ADPRC1
• ADP-ribosyl cyclase 1
• ADP-ribosyl Cyclase
• ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• cADPr hydrolase 1
• CD_antigen: CD38
• CD38 antigen (p45)
• CD38 antigen
• CD38 molecule
• CD38
• cluster of differentiation 38
• Cyclic ADP-ribose hydrolase 1
• Cyclic ADP-ribose Hydrolase
• EC 2.4.99.20
• EC 3.2.2.6
• EC:3.2.2.6
• NAD(+) nucleosidase
• T10

Background

CD38 (cluster of differentiation 38), previously known as T10, is a 46 kDa type II transmembrane glycoprotein (1). CD38 is expressed in both lymphoid and non-lymphoid tissue including in thymocytes, T and B lymphocytes, myeloid cells, natural killer cells, plasma cells, erythrocytes, and additionally in cells of the brain, pancreas, muscle, and bone (1,2). Structurally, CD38 is an "L"-shape which is formed by two separate domains connected by a three peptide-chain hinge region (2). The N-terminal domain is composed of five alpha-helices and two beta strands, while the C-terminal domain contains a four-stranded parallel beta-sheet and two long and two short alpha-helices (2). The CD38 molecule is located on chromosome 4 and is 300 amino acids (aa) in length with a theoretical molecular weight of 34 kDa that functions as both a receptor and an enzyme (1-6). As a receptor, CD38 interacts with its ligand CD31, which is largely expressed in endothelial cells (2-6). As an ectoenzyme, CD38 has a role in calcium signaling and is responsible for the conversion of nicotinamide adenine dinucleotide (NAD) into adenosine diphosphate-ribose (ADPR) or cyclic ADPR and the conversion of phosphorylated NAD (NADP) into nicotinic acid adenine dinucleotide phosphate (NAADP) (2-6).

As described above, CD38 is highly expressed in plasma cells and, as a result, is a target for treating multiple myeloma (MM), the cancer of white blood cells (4,6). The anti-CD38 monoclonal antibody daratumumab is one specific treatment for MM (4,6). Daratumumab has been shown to target MM cells through antibody-dependent cellular cytotoxicity and antibody dependent cellular phagocytosis (4). Additionally, CD38 has a potential role in neurodegenerative disorders and neuroinflammation as elucidated CD38's high expression in neurons, astrocytes, and microglia along with its enzymatic role in NAD degradation (3). Reduced NAD levels is a consequence of aging and occurs during neurodegeneration (3). Furthermore, murine studies have found that CD38 deletion inhibits neuroinflammation and neurodegeneration and therefore might be a potential therapeutic target (3). Similarly, CD38 inhibitors, like quercetin and luteolin, are used to treat age-related diseases and metabolic disorders (7).

References

1. Malavasi, F., Funaro, A., Alessio, M., DeMonte, L. B., Ausiello, C. M., Dianzani, U., Lanza, F., Magrini, E., Momo, M., & Roggero, S. (1992). CD38: a multi-lineage cell activation molecule with a split personality. International journal of clinical & laboratory research. https://doi.org/10.1007/BF02591400

2. Malavasi, F., Deaglio, S., Funaro, A., Ferrero, E., Horenstein, A. L., Ortolan, E., Vaisitti, T., & Aydin, S. (2008). Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiological reviews. https://doi.org/10.1152/physrev.00035.2007

3. Guerreiro, S., Privat, A. L., Bressac, L., & Toulorge, D. (2020). CD38 in Neurodegeneration and Neuroinflammation. Cells. https://doi.org/10.3390/cells9020471

4. van de Donk, N., Richardson, P. G., & Malavasi, F. (2018). CD38 antibodies in multiple myeloma: back to the future. Blood. https://doi.org/10.1182/blood-2017-06-740944

5. Lund, F. E., Cockayne, D. A., Randall, T. D., Solvason, N., Schuber, F., & Howard, M. C. (1998). CD38: a new paradigm in lymphocyte activation and signal transduction. Immunological reviews. https://doi.org/10.1111/j.1600-065x.1998.tb01573.x

6. Glaria, E., & Valledor, A. F. (2020). Roles of CD38 in the Immune Response to Infection. Cells. https://doi.org/10.3390/cells9010228

7. Rajman, L., Chwalek, K., & Sinclair, D. A. (2018). Therapeutic Potential of NAD-Boosting Molecules: The In Vivo Evidence. Cell metabolism. https://doi.org/10.1016/j.cmet.2018.02.011

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD38 Antibody (NBP2-25250)(4)

Publications using NBP2-25250

• Norwood I, Szondi D, Ciocca M et al. Transcriptomic and bioinformatic analysis of Clcn7-dependent Autosomal Dominant Osteopetrosis Type 2 Preclinical and clinical implications Bone Dec 21 2020 [PMID: 33359007] (WB, Mouse)
• Dang T, Liou GY. Macrophage Cytokines Enhance Cell Proliferation of Normal Prostate Epithelial Cells through Activation of ERK and Akt Sci Rep May 16 2018 [PMID: 29769604] (ICC/IF)
• Kang Lei, Jiang Dawei, England Christopher G et al. ImmunoPET imaging of CD38 in murine lymphoma models using 89Zr-labeled daratumumab. European Journal of Nuclear Medicine and Molecular Imaging 2018 Feb 15 [PMID: 29450576] (WB, ICC/IF, Mouse)
• Ma J, Gong W, Liu S et al. Ibrutinib targets microRNA-21 in multiple myeloma cells by inhibiting NF-kB and STAT3 Tumour Biol. 2018 Jan 01 [PMID: 29320977] (Human)

Review for CD38 Antibody (NBP2-25250) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 08/27/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: B cell lymphoma
• Species: Human
• Lot: 061145577F401

Comments

• Comments: Dilution ratio: 1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X
  Blocking details: 10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

FAQs for CD38 Antibody (NBP2-25250). (Showing 1 - 1 of 1 FAQs).

1. Which is your best antibody anti-human CD38 for IHC-P?
   • We do have a number of CD38 antibodies validated in IHC-P, please use the filters on the left side of the search page to help find the product that mostly suits to your experimental design.

Bioinformatics

• Gene Symbol: CD38
• Uniprot:
  • NP_031672(Mouse)