CD36/SR-B3 Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

CD36/SR-B3 Antibody [Alexa Fluor® 405] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
  Gly30-Lys439
  Accession # Q3UAI3
• Specificity: Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD36/SR-B3 Antibody [Alexa Fluor® 405]

• BDPLT10
• CD_antigen: CD36
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 antigen
• CD36 molecule (thrombospondin receptor)
• CD36
• CHDS7
• cluster determinant 36
• Collagen R
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIb
• GPIV
• PAS IV
• PAS-4 protein
• PAS-4
• PASIV
• Platelet collagen receptor
• platelet glycoprotein 4
• SCARB3
• scavenger receptor class B, member 3
• SRB3
• SR-B3
• Thrombospondin R
• Thrombospondin receptor

Background

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.