CD34 Antibody (2936H) [Unconjugated]

PBMC lymphocytes were stained with Mouse Anti-Human CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB1430P) and either (A) Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

CD34 was detected in immersion fixed KG‑1 human acute myelogenous leukemia cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

KG-1a cells were stained with Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 2936H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD34 Antibody (2936H) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CD34
  Ser32-Thr290
  Accession # P28906
• Specificity: Detects human CD34 in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD34 Antibody (2936H) [Unconjugated]

• CD34 antigenhematopoietic progenitor cell antigen CD34
• CD34 molecule
• CD34
• HPCA1

Background

CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

1. Civin C.I. et al. (1984) J. Immunol. 133:157.
2. Katz F. et al. (1985) Leuk. Res. 9:191.
3. Andrews R.G. et al. (1986) Blood 67:842.
4. Young P.E. et al. (1995) Blood 85:96.
5. Krause D.S. et al. (1996) Blood 87:1.
6. Sidney L.E. et al. (2014) Stem Cells 32:1380.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD34 Antibody (MAB11441). (Showing 1 - 1 of 1 FAQ).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Bioinformatics

• Uniprot:
  • P28906()