CD3 zeta Antibody (4D10A6) - BSA Free

Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4D10A6) [NBP1-47337] - Immunofluorescence analysis of peripheral blood T cells using CD3 mouse mAb (A) and flow cytometric analysis of eripheral blood T cells using CD3 mouse mAb (B).

Flow Cytometry: CD3 zeta Antibody (4D10A6) [NBP1-47337] - FCM analysis CD3 expressed in human peripheral blood cells. Image using the Unpurified format of this antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: 4D10A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD3 zeta Antibody (4D10A6) - BSA Free Summary

• Description: Novus Biologicals Mouse CD3 zeta Antibody (4D10A6) - BSA Free (NBP1-47337) is a monoclonal antibody validated for use in ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human CD3 zeta expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD247
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD3 zeta Antibody (4D10A6) - BSA Free

• CD247 antigen
• CD247 antigen, zeta subunit
• CD247 molecule
• CD247
• CD3 zeta
• CD3H
• CD3Q
• CD3z antigen, zeta polypeptide (TiT3 complex)
• CD3Z
• CD3ZT-cell receptor T3 zeta chain
• IMD25
• T3Z
• T-cell antigen receptor complex, zeta subunit of CD3
• T-cell surface glycoprotein CD3 zeta chain
• TCR Zeta Chain
• TCRZCD3-ZETA

Background

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD247
• Entrez:
  • 919(Human)