CD3 zeta Antibody (4A12-F6) - Azide and BSA Free

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of CD247 expression in transfected 293T cell line by CD3Z monoclonal antibody (M01), clone 4A12-F6.Lane 1: CD247 transfected lysate (Predicted MW: 18.7 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of monoclonal antibody to CD3Z on formalin-fixed paraffin-embedded human lymph node tissue. Antibody concentration 5 ug/ml.

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - CD3Z monoclonal antibody (M01), clone 4A12-F6 Analysis of CD3Z expression in Jurkat.

Immunoprecipitation: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of CD247 transfected lysate using anti-CD247 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD247 MaxPab rabbit polyclonal antibody.

ELISA: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Detection limit for recombinant GST tagged CD247 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 4A12-F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD3 zeta Antibody (4A12-F6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CD3Z (AAH25703, 1 a.a. ~ 164 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKWKALFTAAILQAQLPITEAQSFGLLDPKLCYLLDGILFIYGVILTALFLRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR
• Specificity: CD3Z - CD3Z antigen, zeta polypeptide (TiT3 complex)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD247
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CD3 zeta Antibody (4A12-F6) - Azide and BSA Free

• CD247 antigen
• CD247 antigen, zeta subunit
• CD247 molecule
• CD247
• CD3 zeta
• CD3H
• CD3Q
• CD3z antigen, zeta polypeptide (TiT3 complex)
• CD3Z
• CD3ZT-cell receptor T3 zeta chain
• IMD25
• T3Z
• T-cell antigen receptor complex, zeta subunit of CD3
• T-cell surface glycoprotein CD3 zeta chain
• TCR Zeta Chain
• TCRZCD3-ZETA

Background

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD247
• Entrez:
  • 919(Human)
• OMIM:
  • 186780(Human)
• Uniprot:
  • AAH25703(Human)