CD3 epsilon Antibody (BLR174J)

Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Rabbit anti-CD3E recombinant monoclonal antibody [BLR174J] used at 1:1000.

Immunohistochemistry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.

Flow Cytometry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody or isotype control (unshaded).

CD3e was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Rabbit Anti-Human/Mouse CD3e, Monoclonal Antibody (Catalog # NBP3-14747) at 1:4000 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).​ Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR174J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD3 epsilon Antibody (BLR174J) Summary

• Description: Novus Biologicals Rabbit CD3 epsilon Antibody (BLR174J) (NBP3-14747) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: residues 139 and 189 (C-term)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD3E
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 12 µl/1 mg lysate
  • Western Blot 1:1,000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for CD3 epsilon Antibody (BLR174J)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E