CD3 Antibody (OKT3) - Low Endotoxin and Azide Free


Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis of CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody more
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) and isotype control (green) at 0.2 ug/10^6 cells.
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) grown in serum-free media and isotype control (green) at 0.25 ug/10^6 cells.
Flow (Cell Surface): CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 more
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-24867. Staining of human PBMC. Image from verified customer review.
Flow (Cell Surface): CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the PE conjugate of NBP2-24867. Detection of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/ml. Cells were more
Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications Flow, Flow-CS, Func, In vivo, CyTOF-ready
Low Endotoxin and Azide Free
1.0 mg/ml

CD3 Antibody (OKT3) - Low Endotoxin and Azide Free Summary

Human PBMCs were used as immunogen.
IgG2a Kappa
Protein G purified
Endotoxin Note
Endotoxin amounts will vary lot to lot, but should be less than 0.03 EU/ug.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1ul/1 million cells
  • Functional
  • In vivo assay
  • CyTOF-ready
  • Flow (Cell Surface)
Application Notes
Use in In-vivo reported in scientific literature (PMID 25535359). Use in In vitro reported in scientific literature (PMID 26084721)
Read Publications using
NBP2-24867 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25485679)

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Filter sterilized PBS.
No Preservative
1.0 mg/ml
Protein G purified

Alternate Names for CD3 Antibody (OKT3) - Low Endotoxin and Azide Free

  • anti-CD3 okt3
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • Muromonab
  • okt3 ab
  • okt3 CD3
  • okt3 hybridoma
  • okt3
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE


CD3 (T3), a complex Tcell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the Tcell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. Structurally, CD3 consists of four distinct membrane glycoprotein isoforms (CD3-g, CD3-e, CD3-s and the zeta chain) to generate activation signals. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for Tcell development in the thymus, proliferation, and induction of Tcell-mediated immune responses against infectious agents and also in the differentiation of Tcells into effector and memory populations. It usually expresses in the cytoplasm of prothymocytes and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukaemia, CD3 can also be useful for the identification of Tcells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. It indirectly plays an important role in immunomodulation whereas the CD3 antibody may be used in in vitro Treg assays to generate effector Tcells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (NBP2-24867)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, Func, In vitro, In vivo.

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Showing Publications 1 - 8 of 8.
Publications using NBP2-24867 Applications Species
Ma SD, Xu X, Jones R et al. PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse Model. PLoS Pathog. May 1 2016 [PMID: 27186886] (In vivo, Mouse)

CD3 antibody (clone OKT3) was used for in vivo i.p. treatment of mice (50 ug of antibody three times per week) starting eleven days after EBV infection and continuing for the remainder of the experiment.
In vivo Mouse
Whitehurst CB, Li G, Montgomery SA et al. Knockout of Epstein-Barr Virus BPLF1 Retards B-Cell Transformation and Lymphoma Formation in Humanized Mice. MBio. 2015 Oct 22 [PMID: 26489865] (In vivo, Human) In vivo Human
Kim SG, Lowe EL, Dixit D et al. Cocaine-mediated impact on HIV infection in humanized BLT mice Sci Rep. 2015 Jun 18 [PMID: 26084721] (In vitro, Func, Human)

CD3 antibody (clone OKT3) was used at 50ng/ml concentration for the activation of CD8+?T cells which were isolated from PBMC of healthy human donors.
In vitro, Func Human
Ma SD, Xu X, Plowshay J et al. LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis. J. Clin. Invest. 2014 Dec 08 [PMID: 25535359] (In vivo)

CD3 antibody (clone OKT3) injected into hNSG(thy) mice (NSG mice engrafted with both human fetal hematopoietic stem cells and thymic tissue) at 2 ug/d dose starting 1 day after EBV infection and continuing for 6 days per week (alternating i.p. and i.v. route) - OKT3's increased the number and size of EBV-infected lymphomas in this humanized mouse model (Figure 11A)
In vivo
Ma SD Xu X Plowshay J et al. LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis. J Clin Invest. [PMID: 25485679] (In vivo, Mouse) In vivo Mouse
Gill T, Levine AD. Mitochondria-derived hydrogen peroxide selectively enhances T cell receptor-initiated signal transduction. J Biol Chem. 2013 Sep 6 [PMID: 23880762]
Ma SD, Yu X, Mertz JE et al. An Epstein-Barr Virus (EBV) mutant with enhanced BZLF1 expression causes lymphomas with abortive lytic EBV infection in a humanized mouse model. J Virol. 2012 Aug [PMID: 22623780] (Mouse)

CD3 (IMG-6240E). FA: Mice received 2 ug of CD3 antibody intravenously per day, starting 1 day after EBV infection, Fig 1A & Fig 1B.
Crellin NK Garcia RV Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. [PMID: 16339542] (FLOW, Human) FLOW Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD3 Antibody (NBP2-24867). (Showing 1 - 2 of 2 FAQs).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.
  2. Has this antibody been tested for the presence of any virus?
    • Unfortunately all of Novus' antibodies, including NBP2-24867, are just for laboratory research uses, not for any clinical diagnostics or therapeutic treatment. Therefore this antibody has not been tested for the presence of any virus, as required by the rules of GMP for all clinical products.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24867AF405
Alexa Fluor 488 Labeled NBP2-24867AF488
Alexa Fluor 647 Labeled NBP2-24867AF647
Alexa Fluor 700 Labeled NBP2-24867AF700
Allophycocyanin Labeled NBP2-24867APC
DyLight 350 Labeled NBP2-24867UV
DyLight 405 Labeled NBP2-24867V
DyLight 488 Labeled NBP2-24867G
DyLight 550 Labeled NBP2-24867R
DyLight 650 Labeled NBP2-24867C
DyLight 680 Labeled NBP2-24867FR
DyLight 755 Labeled NBP2-24867IR
PE Labeled NBP2-24867PE
PerCP Labeled NBP2-24867PCP

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Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
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Gene Symbol CD3E