CD3 Antibody (OKT3) [Alexa Fluor® 700]

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Flow Cytometry: CD3 Antibody (OKT3) [Alexa Fluor (R) 700] [NBP2-24867AF700] - Flow cytometry on human PBMC. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, Flow
Clone
OKT3
Clonality
Monoclonal
Host
Mouse

CD3 Antibody (OKT3) [Alexa Fluor® 700] Summary

Immunogen
Human PBMCs were used as immunogen.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD3
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 700
Format
Alexa Fluor 700 (A=675-700, E=723)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing
  • Flow Cytometry 1ul/1 million cells
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24867AF700 in the following applications:

Notes

Alexa Flour (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for CD3 Antibody (OKT3) [Alexa Fluor® 700]

  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for CD3 Antibody (NBP2-24867AF700) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-24867AF700:
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Images Ratings Applications Species Date Details
Flow Cytometry CD3 NBP2-24867AF700
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reviewed by:
Anon ymous
Flow Human 09/17/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman
LotAB121609A-AF700

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD3 Antibody (NBP2-24867AF700). (Showing 1 - 1 of 1 FAQ).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.

Secondary Antibodies

 

Isotype Controls

Additional CD3 Products

CD3 NBP2-24867AF700

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Blogs on CD3.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CD3
Entrez

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