CD25/IL-2R alpha Antibody (1037230) [Unconjugated]

Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10202). The ND50 is typically 0.2-2.0 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 1037230
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD25/IL-2R alpha Antibody (1037230) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human CD25/IL-2R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2R alpha in ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (1037230) [Unconjugated]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 receptor alpha (IL-2R alpha), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit (gamma c). Human IL-2R alpha cDNA encodes a 213 amino acid (aa) precursor with a 21 aa signal peptide and a 192 aa extracellular region. The ECD of Human IL-2R alpha shares a 59% amino acid sequence identity with the ECD of mouse and rat IL-2R alpha, respectively. IL‑2R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). IL-2R beta (CD122) and gamma c (IL-2R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (4, 5). By itself, IL-2R alpha binds IL-2 with low affinity. IL-2R alpha makes no contacts with IL-2R beta or gamma c, and only minor changes are observed in the IL-2 structure in response to receptor binding. These findings support the principal role of IL-2R alpha to deliver IL-2 to the signaling complex and act as regulator of signal transduction (6, 7). A soluble form of IL‑2R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2R alpha are found in some cancers and immune disorders (10). IL-2R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2R alpha is also required for the development of CD4+CD25+ Treg which suppresses autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
4. Hatakeyama, M. et al. (1989) Science 244:551.
5. Takeshita, T. et al. (1992) Science 257:379.
6. Stauber, D. et al. (2006) Proc. Natl. Acad. Sci. U. S. A. 103:2788.
7. Wang, X. et al. (2005) Science 310:1159.
8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
11. Willerford, D.M. et al. (1995) Immunity 3:521.
12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01589()