CD21 Antibody (LB21)

Flow Cytometry: CD21 Antibody (LB21) [NB100-65323] - Analysis using the FITC conjugate of NB100-65323. Staining of human peripheral blood lymphocytes.

Flow Cytometry: CD21 Antibody (LB21) [NB100-65323] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD21.

Product Discontinued

CD21 Antibody (LB21) Summary

• Immunogen: Human IM9 cell line.
• Localization: Type I membrane protein
• Marker: Histiocytic Marker
• Specificity: NB100-65323 is a 14kD cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunoprecipitation 1:10-1:500
• Application Notes: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Reactivity Notes

Reacts with Human. Cross reacts with Sheep, Mink, Bovine, Goat and Cat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.025 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD21 Antibody (LB21)

• C3DR
• C3DRSLEB9
• CD21 antigen
• CD21
• Complement C3d receptor
• complement component (3d/Epstein Barr virus) receptor 2
• complement receptor type 2
• CR2
• EBV receptor
• Epstein-Barr virus receptor

Background

CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CR2
• Entrez:
  • 1380(Human)
• Uniprot:
  • P20023(Human)