CD21 Antibody (BB6-11C9.6) - Azide and BSA Free

Flow Cytometry: CD21 Antibody (BB6-11C9.6) [NBP1-28245] - Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD21-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.

Flow Cytometry: CD21 Antibody (BB6-11C9.6) [NBP1-28245] - Analysis using the Biotin conjugate of NBP1-28245. Multiple staining of peripheral blood lymphocytes.

Flow Cytometry: CD21 Antibody (BB6-11C9.6) [NBP1-28245] - Analysis using the FITC conjugate of NBP1-28245. Multiple staining of peripheral blood lymphocytes.

• Reactivity: Mu, Po
• Applications: Flow
• Clone: BB6-11C9.6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CD21 Antibody (BB6-11C9.6) - Azide and BSA Free Summary

• Immunogen: Porcine CD21, Mr 160 kDa
• Marker: Histiocytic Marker
• Specificity: Porcine CD21, Mr 160 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26284585). Use in Mouse reported in scientific literature (PMID:26284585).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD21 Antibody (BB6-11C9.6) - Azide and BSA Free

• C3DR
• C3DRSLEB9
• CD21 antigen
• CD21
• Complement C3d receptor
• complement component (3d/Epstein Barr virus) receptor 2
• complement receptor type 2
• CR2
• EBV receptor
• Epstein-Barr virus receptor

Background

CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. (1) Low density CD21 has also been reported on granulocytes and monocytes. MAb BB6-11C9. (6) stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer's patches (ILPP); 93-97% in spleen; and 0% in thymus. (2) The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation). (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD21 Antibody (NBP1-28245)(3)

Publications using NBP1-28245

• Ramm R, Goecke T, KOhler P Et al. Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs Regen Biomater 2022-08-05 [PMID: 35928180]
  
  Details:
  Citation using the Biotin version of this antibody.
• Kick AR, Wolfe ZC, Amaral AF et al. Maternal Autogenous Inactivated Virus Vaccination Boosts Immunity to PRRSV in Piglets Vaccines 2021-01-31 [PMID: 33572562] (FLOW, Porcine)
• John-Schuster G, Gunter S, Hager K et al. Inflammaging increases susceptibility to cigarette smoke-induced COPD. Oncotarget. 2016-05-24 [PMID: 26284585] (Mouse)
  
  Details:
  This citation used the Alexa Fluor 647 version of this antibody.

Bioinformatics

• Gene Symbol: CR2