CD20 Antibody (L26)

Images

 
Western Blot: CD20 Antibody (L26) [NBP2-44743] - Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).
Immunocytochemistry/ Immunofluorescence: CD20 Antibody (L26) [NBP2-44743] - Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-Mouse IgG conjugated to CF488 ...read more
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (L26) [NBP2-44743] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26)
Flow Cytometry: CD20 Antibody (L26) [NBP2-44743] - Cytometric Analysis of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (L26) [NBP2-44743] - Human Tonsil stained with CD20 Monoclonal Antibody (L26)

Product Details

Summary
Reactivity Hu, Pm, Pm, Bv(-), Ca(-), Po(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
L26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD20 Antibody (L26) Summary

Immunogen
Human tonsil B cells
Localization
Predominantly cell surface with some cytoplasmic
Marker
B-Cell Marker
Specificity
Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkins disease, particularly of lymphocyte predominant type.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MS4A1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 2 - 5ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunofluorescence 1:100-1:200
Application Notes
Hu-chromosome location: 11q12.2 Molecular weight of antigen: 33-37kDa Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. Use in Immunohistochemistry reported in scientific literature (PMID: 29669578). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-44743 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD20 Antibody (L26)

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD20 Antibody (NBP2-44743)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD20 Antibody (NBP2-44743) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-80486AF350
Alexa Fluor 405 NBP2-80486AF405
Alexa Fluor 488 NBP2-80486AF488
Alexa Fluor 532 NBP2-80486AF532
Alexa Fluor 594 NBP2-80486AF594
Alexa Fluor 647 NBP2-80486AF647
Alexa Fluor 700 NBP2-80486AF700
Alexa Fluor 750 NBP2-80486AF750
Allophycocyanin NBP2-80486APC
Biotin NBP2-80486B
DyLight 350 NBP2-80486UV
DyLight 405 NBP2-80486V
DyLight 488 NBP2-80486G
DyLight 550 NBP2-80486R
DyLight 594 NBP2-80486DL594
DyLight 650 NBP2-80486C
DyLight 680 NBP2-80486FR
DyLight 755 NBP2-80486IR
FITC NBP2-80486F
HRP NBP2-80486H
Janelia Fluor 549 NBP2-80486JF549
Janelia Fluor 646 NBP2-80486JF646
PE NBP2-80486PE
PerCP NBP2-80486PCP

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Antigen-loss relapse after successful CAR-T therapy; What do we do now?
By Jacqueline Carrico, BS, MD Tumor cell mechanisms driving tolerance to CAR-T Despite very promising results of CAR-T therapy in acute lymphoblastic leukemia, B-ALL, antigen-loss relapse has arisen as a major chall...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
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Bioinformatics

Gene Symbol MS4A1
Uniprot