CD163 Antibody (215927) [Unconjugated]

PBMC were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB1607) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 215927
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD163 Antibody (215927) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD163
  Gly46-Ser1050
  Accession # Q86VB7
• Specificity: Detects human CD163 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD163
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD163 Antibody (215927) [Unconjugated]

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
4. Kristiansen, M. et al. (2001) Nature 409:198.
5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
6. Moller, H.J. et al. (2002) Blood 99:378.
7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
13. Schaer, D.J. et al. (2006) Blood 107:373.
14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
15. Fabriek, B.O. et al. (2007) Blood 109:5223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD163 Antibody (MAB1607)(13)

Publications using MAB1607

• Helen Ferreira, Mayara Abud Mendes, Mayara Garcia de Mattos Barbosa, Eliane Barbosa de Oliveira, Anna Maria Sales, Milton Ozório Moraes et al. Potential Role of CXCL10 in Monitoring Response to Treatment in Leprosy Patients Frontiers in Immunology 2021-07-20 [PMID: 34354699]
• M Prat, M Salon, T Allain, O Dubreuil, G Noël, L Preisser, B Jean, L Cassard, F Lemée, I Tabah-Fish, B Pipy, P Jeannin, JF Prost, JM Barret, A Coste Murlentamab, a Low Fucosylated Anti-M�llerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation Cancers, 2021-04-13;13(8):. 2021-04-13 [PMID: 33924378] (Flow Cytometry, Human)
• C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling PLoS ONE, 2021-04-22;16(4):e0250107. 2021-04-22 [PMID: 33886609] (ICC, Human)
• Tatiana Pereira da Silva, Tamiris Lameira Bittencourt, Ariane Leite de Oliveira, Rhana Berto da Silva Prata, Vinicius Menezes, Helen Ferreira et al. Macrophage Polarization in Leprosy–HIV Co-infected Patients Frontiers in Immunology 2020-07-29 [PMID: 32849508] (Immunohistochemistry, Human)
• C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth Cancer Res., 2018-09-05;0(0):. 2018-09-05 [PMID: 30185551] (Flow Cytometry, Human)
• N McGovern, A Shin, G Low, D Low, K Duan, LJ Yao, R Msallam, I Low, NB Shadan, HR Sumatoh, E Soon, J Lum, E Mok, S Hubert, P See, EH Kunxiang, YH Lee, B Janela, M Choolani, CNZ Mattar, Y Fan, TKH Lim, DK Hong, KK Tan, JKC Tam, C Schuster, A Elbe-Bürge, XN Wang, V Bigley, M Collin, M Haniffa, A Schlitzer, M Poidinger, S Albani, A Larbi, EW Newell, JKY Chan, F Ginhoux Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2 Nature, 2017-06-14;0(0):. 2017-06-14 [PMID: 28614294] (Flow Cytometry, Human)
• Jagged1 Instructs Macrophage Differentiation in Leprosy PLoS Pathog, 2016-08-17;12(8):e1005808. 2016-08-17 [PMID: 27532668] (Flow Cytometry, Human)
• Cuilian Dai, Xianglan Yao, Elizabeth M. Gordon, Amisha Barochia, Rosemarie A. Cuento, Maryann Kaler et al. A CCL24-dependent pathway augments eosinophilic airway inflammation in house dust mite-challenged Cd163(-/-) mice Mucosal Immunology 2016-05-01 [PMID: 26376364]
• Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor. Cancer Res, 2012-09-12;72(22):5733-43. 2012-09-12 [PMID: 22971345] (ICC, Human)
• Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype. Clin. Cancer Res., 2011-04-13;17(14):4642-9. 2011-04-13 [PMID: 21490182] (Flow Cytometry, Human)
• Thelen T, Hao Y, Medeiros AI The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages. J. Immunol., 2010-09-01;185(7):4328-35. 2010-09-01 [PMID: 20810988] (Neutralization, Human)
• Ochiel DO, Ghosh M, Fahey JV Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands. J. Leukoc. Biol., 2010-04-12;88(3):435-44. 2010-04-12 [PMID: 20385795] (Flow Cytometry, Human)
• Choi KD, Vodyanik MA, Slukvin II Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors. J. Clin. Invest., 2009-08-10;119(9):2818-29. 2009-08-10 [PMID: 19726877] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD163
• Uniprot:
  • Q86VB7()