CD16/CD32 Antibody (93) - Azide and BSA Free

Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-27946. Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2a K Isotype Control PerCP-Cy5.5 (open histogram) or 0.06 ug of Anti-Mouse CD16/CD32 PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.

Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Staining of mouse splenocytes with Anti-Mouse CD16/CD32 APC (left), and PE-Cy7 (right). Autofluorescence is shown via open histogram. Total cells were used for analysis.

Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Analysis using the PE conjugate of NBP1-27946. Multiple staining of BALB/c splenocytes.

Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Analysis using the PE/Cy7 conjugate of NBP1-27946. Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 ug of Anti-Mouse CD16/CD32 PE-Cy7 (filled histogram). Total cells were used for analysis.

• Reactivity: Mu
• Applications: Flow, IHC, IHC-Fr, IP, B/N
• Clone: 93
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

CD16/CD32 Antibody (93) - Azide and BSA Free Summary

• Immunogen: Murine CD16/32 (CD16/Fc gamma III and CD32/FcgammaII receptors)
• Specificity: Murine CD16/32 (CD16/Fc gamma III and CD32/FcgammaII receptors)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FCGR3A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry <= 3 ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD16/CD32 Antibody (93) - Azide and BSA Free

• CD16
• CD16A
• Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• FCG3
• FCGR3
• FCGR3A
• FCGRIII
• FCR-10
• FCRIII
• FCRIIIA
• IGFR3

Background

The lymphocyte Fcgamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. (1, 2) The Fcgamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. (1) The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying FcgammaR+ cells, monoclonal antibodies to the FcgammaII/III receptor have been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcgammaR for parasitic invasion. (4-6) The monoclonal antibody 93 recognizes a conformational epitope formed by FcgammaII and FcgammaIII receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR3A