CD14 Antibody [Biotin]

CD14 was detected in immersion fixed paraffin-embedded sections of human lymph node using 10 µg/mL Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-AEC Cell & Tissue Staining Kit (red; CTS020) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CD14 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (Positive) and absent in MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) using Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD14 was detected in immersion fixed U937 human histiocytic lymphoma cell line (Positive) and absent in MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) using Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

CD14 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD14
  Thr20-Cys352
  Accession # P08571
• Specificity: Detects human CD14 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse CD14 and Lipopolysaccharide is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CD14
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CD14 Antibody (Catalog # MAB3833)
  ELISA Detection: Human CD14 Biotinylated Antibody (Catalog # BAF383)
  Standard: Recombinant Human CD14 (Catalog # 383-CD)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD14 Antibody [Biotin]

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

1. Wright, S.D. et al. (1990) Science 249:1431.
2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
4. Stelter, F. (2000) Chem. Immunol. 74:25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD14 Antibody (BAF383)(14)

Publications using BAF383

• Kraaijvanger, R;Janssen Bonás, M;Grutters, JC;Paspali, I;Veltkamp, M;de Kleijn, DPV;van Moorsel, CHM; Decreased serpin C1 in extracellular vesicles predicts response to methotrexate treatment in patients with pulmonary sarcoidosis Respiratory research 2024-04-16 [PMID: 38627696] (ELISA Detection, Human)
• Larionova I, Patysheva M, Iamshchikov P et al. PFKFB3 overexpression in monocytes of patients with colon but not rectal cancer programs pro-tumor macrophages and is indicative for higher risk of tumor relapse Frontiers in immunology 1/17/2023 [PMID: 36733385]
  
  Details:
  1:100 IHC-P dilution
• Marina Patysheva, Irina Larionova, Marina Stakheyeva, Evgeniya Grigoryeva, Pavel Iamshchikov, Natalia Tarabanovskaya et al. Effect of Early-Stage Human Breast Carcinoma on Monocyte Programming Frontiers in Oncology 2/14/2022 [PMID: 35237501]
• WM Gama, CHM Frank, TVR Almeida, DS Dos Santos, YO Chaves, DF da Silva, PP Orlandi, FR Pereira, GF Magalhães, BJ Baptista, VL de Oliveir, AA da Silva B, MF Santana, RL Gonçalves, AG da Costa, MC Dos Santos, LC de Lima Fe, MVG Lacerda, PA Nogueira Immunologic biomarkers, morbidity and mortality among HIV patients hospitalised in a Tertiary Care Hospital in the Brazilian Amazon BMC infectious diseases, 2021-08-26;21(1):876. 2021-08-26 [PMID: 34445956] (ELISA Development, Human)
• B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis PLoS ONE, 2020-06-03;15(6):e0234038. 2020-06-03 [PMID: 32492075] (IHC, Human)
• Greiffo FR, Viteri-Alvarez V, Frankenberger M et al. CX3CR1-fractalkine axis drives kinetic changes of monocytes in fibrotic interstitial lung diseases Eur. Respir. J. 11/19/2019 [PMID: 31744836]
• SA Aschenbroi, ER Lafontaine, MC Lopez, HV Baker, RJ Hogan Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms BMC Med Genomics, 2019-09-06;12(1):127. 9/6/2019 [PMID: 31492148]
• X Hou, G Chen, W Bracamonte, HS Choi, NL Diny, J Sung, D Hughes, T Won, MK Wood, MV Talor, DJ Hackam, K Klingel, G Davogustto, H Taegtmeyer, I Coppens, JG Barin, D ?iháková The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis Cell Rep, 2019-07-02;28(1):172-189.e7. 2019-07-02 [PMID: 31269438] (IHC, Human)
• Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort. PLoS ONE, 2016-01-28;11(1):e0148073. 2016-01-28 [PMID: 26820481] (ELISA Development, Human)
• Jafari A, Siersbaek M, Chen L, Qanie D, Zaher W, Abdallah B, Kassem M Pharmacological Inhibition of Protein Kinase G1 Enhances Bone Formation by Human Skeletal Stem Cells Through Activation of RhoA-Akt Signaling. Stem Cells, 2015-04-23;33(7):2219-31. 2015-04-23 [PMID: 25858613] (Cell Selection, Human)
• Komori M, Blake A, Greenwood M et al. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis. Ann Neurol 7/1/2015 [PMID: 25808056]
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Blais DR, Harrold J, Altosaar I Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract. Pediatr. Res., 2006-03-01;59(3):371-6. 2006-03-01 [PMID: 16492974] (Western Blot, Human)
• Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaisse JM Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation. Cancer Res., 2005-11-01;65(21):9943-52. 2005-11-01 [PMID: 16267019] (Affinity Purification, Human)

Bioinformatics

• Gene Symbol: CD14
• Uniprot:
  • P08571()