CD-M6PR Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD-M6PR Lumenal Domain at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD-M6PR Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD-M6PR Lumenal Domain
  Thr27-His185
  Accession # P20645
• Specificity: Detects human CD-M6PR Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse CD-M6PR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: M6PR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD-M6PR Antibody

• 000 Man6PR
• 46 kDa mannose 6-phosphate receptor
• 46-kDa mannose 6-phosphate receptor
• cation-dependent mannose-6-phosphate receptor
• CD Man-6-P receptor
• CDM6PR
• CD-M6PR
• CD-MPR
• FLJ32994
• M6PR
• mannose-6-phosphate receptor (cation dependent)
• MPR 46
• MPR46
• MPRD
• Mr 46
• SMPR

Background

The CD-M6PR (cation-dependent mannose 6-phosphate receptor; also CD-MPR) is a 44-46 kDa member of the P-type lectin family of proteins. It is ubiquitously expressed, found internally, and transports newly formed mannose 6-phosphate bearing acid hydrolases from the secretory pathway to acidified lysosomes. Mature human CD-M6PR is a type I transmembrane glycoprotein that is 251 amino acids (aa) in length. It functions as a homodimer, and contains a 159 aa luminal region (aa 27-185) plus a 67 aa cytoplasmic domain. The aa residues Gln66, Arg111, Glu133 and Tyr143 recognize carbohydrate; Asp103 interacts with divalent cation (Mn2 + ), increasing ligand affinity and receptor oligomerization. Over aa 27-184, human CD-M6PR shares 90% aa identity with mouse CD-M6PR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD-M6PR Antibody (AF5320)(1)

Publication using AF5320

• H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease Acta Neuropathol Commun, 2019-01-31;7(1):13. 2019-01-31 [PMID: 30704515] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: M6PR
• Uniprot:
  • P20645()