CCR9 Antibody (242503) [Unconjugated]

Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB2160, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 242503
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR9 Antibody (242503) [Unconjugated] Summary

• Immunogen: Y3 rat myeloid cell line transfected with mouse CCR9
  Met1-Leu369
  Accession # Q9WUT7
• Specificity: Detects mouse CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR9 Antibody (242503) [Unconjugated]

• C-C chemokine receptor type 9
• C-C CKR-9
• CC-CKR-9
• CCR9
• CCR-9
• CD199
• CDw199 antigen
• CDw199
• chemokine (C-C motif) receptor 9
• G protein-coupled receptor 28
• GPR28
• GPR-9-6G-protein coupled receptor 28

Background

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR9 Antibody (MAB2160)(9)

Publications using MAB2160

• Martini S, Davis K, Faraway R Et al. A genetically-encoded crosslinker screen identifies SERBP1 as a PKCepsilon substrate influencing translation and cell division Nature communications 11/26/2021 [PMID: 34836941]
• BA Szilagyi, J Triebus, C Kressler, M de Almeida, S Tierling, P Durek, M Mardahl, A Szilagyi, S Floess, J Huehn, U Syrbe, J Walter, JK Polansky, A Hamann Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4(+) memory T cells Mucosal Immunol, 2017-02-15;0(0):. 2017-02-15 [PMID: 28198363] (Flow Cytometry, Mouse)
• Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties Sci Rep, 2016-11-29;6(0):37914. 2016-11-29 [PMID: 27897208] (Flow Cytometry, Mouse)
• Bakdash G, Vogelpoel L, Van Capel T, Kapsenberg M, de Jong E Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells. Mucosal Immunol, 2014-07-16;8(2):265-78. 2014-07-16 [PMID: 25027601] (Flow Cytometry, Mouse)
• Heidegger S, Kirchner S, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Russmann H, Endres S, Bourquin C TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice. J Immunol, 2013-04-15;190(10):5313-20. 2013-04-15 [PMID: 23589622] (Flow Cytometry, Mouse)
• Dias S, Mansson R, Gurbuxani S, Sigvardsson M, Kee BL E2A proteins promote development of lymphoid-primed multipotent progenitors. Immunity, 2008-07-31;29(2):217-27. 2008-07-31 [PMID: 18674933] (Flow Cytometry, Mouse)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-09-15;179(6):3724-33. 2007-09-15 [PMID: 17785809] (Flow Cytometry, Mouse)
• Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects. J. Leukoc. Biol., 2006-10-19;81(1):176-85. 2006-10-19 [PMID: 17053165] (Flow Cytometry, Mouse)
• Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization. Blood, 2006-06-29;108(8):2531-9. 2006-06-29 [PMID: 16809609] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR9
• Uniprot:
  • Q9WUT7()