CCR8 Antibody (191704) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin aM/CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB16991G) and either (A) Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Rat splenocytes were stained with Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Rt
• Applications: Flow
• Clone: 191704
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

CCR8 Antibody (191704) [Phycoerythrin] Summary

• Additional Information: Clone 191704 was used by HLDA to establish CD designation
• Immunogen: BaF3 mouse pro-B cell line transfected with human CCR8
  Met1-Leu355
  Accession # P51685
• Specificity: Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR8 Antibody (191704) [Phycoerythrin]

• CC chemokine receptor 8
• C-C CKR-8
• CC-chemokine receptor chemr1
• CC-CKR-8
• CCR8
• CCR-8
• CD198
• CDw198 antigen
• CDw198
• chemokine (C-C motif) receptor 8
• chemokine (C-C) receptor 8
• chemokine (C-C) receptor-like 2
• Chemokine receptor-like 1
• ChemR1
• CKRL1
• CKR-L1
• CKRL1MGC129966
• CMKBR8MGC129973
• CMKBRL2C-C chemokine receptor type 8
• CY6
• GPRCL1
• GPRCY6
• GPR-CY6
• TER-1
• TER1CC chemokine receptor CHEMR1

Background

CCR8 (C-C Chemokine Receptor 8), previously called CKR-L1 or TER1 and designated CD198, is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR8 Antibody (FAB1429P)(5)

Publications using FAB1429P

• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Carbone T, Nasorri F, Pennino D CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. J. Immunol., 2009-12-11;184(2):1102-10. 2009-12-11 [PMID: 20008290] (Flow Cytometry, Human)
• Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES. J. Invest. Dermatol., 2009-01-01;129(6):1569-81. 2009-01-01 [PMID: 19122644] (Flow Cytometry, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR8
• Uniprot:
  • P51685()