CCR7 Antibody (150503R-FAB2-TEV/His) [Unconjugated]

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR7 FAB2 Recombinant Monoclonal Antibody (Catalog # MAB197R-FAB2) or (B) Mouse IgG2A isotype control antibody (MAB003), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR7 FAB2 Recombinant Monoclonal Antibody (Catalog # MAB197R-FAB2) or (B) Mouse IgG2A isotype control antibody (MAB003), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 150503R-FAB2-TEV/His
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCR7 Antibody (150503R-FAB2-TEV/His) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human CCR7 transfectants
  Met1-Pro378
  Accession # AAA58615
• Specificity: Stains human CCR7 in flow cytometry
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR7 Antibody (150503R-FAB2-TEV/His) [Unconjugated]

• BLR2
• BLR2C-C chemokine receptor type 7
• C-C CKR-7
• CC-CKR-7
• CCR7
• CCR-7
• CD197 antigen
• CD197
• CDw197
• CDw197CC chemokine receptor 7
• chemokine (C-C motif) receptor 7
• CMKBR7
• CMKBR7chemokine (C-C) receptor 7
• EBI1
• EBI1EBV-induced G protein-coupled receptor 1
• EBV-induced G-protein coupled receptor 1
• Epstein-Barr virus induced gene 1
• Epstein-Barr virus induced G-protein coupled receptor
• Epstein-Barr virus-induced G-protein coupled receptor 1
• EVI1
• lymphocyte-specific G protein-coupled peptide receptor
• MIP-3 beta receptor

Background

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAA58615()