CCR5 Antibody (12D1)

Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) [NBP2-44483] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).

Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) [NBP2-44483] - Human Stomach tissue stained with CCR5 Antibody (12D1).

Flow Cytometry: CCR5 Antibody (12D1) [NBP2-44483] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: 12D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CCR5 Antibody (12D1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47759)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human native CCR5 protein (Uniprot: P51681)
• Localization: Cell surface
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CCR5 Antibody (12D1)

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

C-C chemokine receptor type 5 (CCR5) is a member of the beta chemokine receptors family of integral membrane proteins. The CCR5 protein is a G protein-coupled receptor which functions as a chemokine receptor in the CC chemokine group.

CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.

CCR5 antibodies are useful for HIV infection studies and research on immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR5
• Uniprot:
  • P51681()
  • P51681()
  • P51681()
  • P51681()