CCR3 Antibody (83101) [Fluorescein]

Mouse splenocytes were stained with Rat Anti-Mouse CCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB729F) and Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Gal-3 deficient Eos exhibit decreased migration toward eotaxin-1. (A) Migration of WT and Gal-3-/- BM-derived Eos toward murine eotaxin-1 (100 nM) in vitro using 96-well Transwell ® Chambers. Results are represented as percent cell migration relative to WT Eos. (B) Expression of CCR3 by WT and Gal-3-/- Eos by flow cytometry using FITC-conjugated rat anti-mouse CCR3 with FITC-conjugated rat IgG2a as isotype control. Representative data of two independent experiments with Eos from different mice is shown. (C) Migration of WT Eos suspended in medium alone or medium containing lactose or maltose toward murine eotaxin-1. Number of cells that migrated in each case was determined and expressed as the average number of cells/field. Combined data (mean ± SEM) from three independent experiments in duplicate is shown in (A) and (C). #p <0.05 versus WT Eos in (A). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2013.00037/abstract), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: Flow
• Clone: 83101
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

CCR3 Antibody (83101) [Fluorescein] Summary

• Immunogen: Y3 rat myeloid cell line transfected with mouse CCR3
  Met1-Phe359
  Accession # P51678
• Specificity: Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR3 Antibody (83101) [Fluorescein]

• b-chemokine receptor
• CC chemokine receptor 3
• C-C chemokine receptor type 3
• C-C CKR-3
• CCR3
• CCR-3
• CD193 antigen
• CD193
• chemokine (C-C motif) receptor 3
• CKR3CC-CKR-3CMKBR3CD193
• eosinophil CC chemokine receptor 3
• Eosinophil eotaxin receptor
• MGC102841

Background

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR3 Antibody (FAB729F)(27)

Publications using FAB729F

• Lee J, Kim SJ, Choi GE et al. Sweet taste receptor agonists attenuate macrophage IL-1 beta expression and eosinophilic inflammation linked to autophagy deficiency in myeloid cells Clinical and translational medicine 8/1/2022 [PMID: 35988262]
  
  Details:
  Dilution used 1:400
• N Iwasaki, S Terawaki, K Shimizu, D Oikawa, H Sakamoto, K Sunami, F Tokunaga Th2 cells and macrophages cooperatively induce allergic inflammation through histamine signaling PLoS ONE, 2021-03-04;16(3):e0248158. 2021-03-04 [PMID: 33662037] (Flow Cytometry, IHC, Mouse)
• M Dileepan, XN Ge, I Bastan, YG Greenberg, Y Liang, P Sriramarao, SP Rao Regulation of Eosinophil Recruitment and Allergic Airway Inflammation by Tropomyosin Receptor Kinase A J. Immunol., 2019-12-23;0(0):. 2019-12-23 [PMID: 31871023] (Flow Cytometry, Mouse)
• Koubun Yasuda, Takumi Adachi, Atsuhide Koida, Kenji Nakanishi Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung Frontiers in Immunology 9/19/2018 [PMID: 30283458]
• I Bastan, XN Ge, M Dileepan, YG Greenberg, AG Guedes, SH Hwang, BD Hammock, RJ Washabau, SP Rao, P Sriramarao Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation J. Leukoc. Biol., 2018-01-17;0(0):. 2018-01-17 [PMID: 29345370] (Flow Cytometry, Mouse)
• P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production Sci Rep, 2017-07-19;7(1):5922. 2017-07-19 [PMID: 28725048] (Flow Cytometry, Mouse)
• SI Ochkur, AD Doyle, EA Jacobsen, WE LeSuer, W Li, CA Protheroe, KR Zellner, D Colbert, HH Shen, CG Irvin, JJ Lee, NA Lee Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airways dysfunction with type 2 inflammation J. Leukoc. Biol., 2017-05-17;0(0):. 2017-05-17 [PMID: 28515227] (Flow Cytometry, Mouse)
• Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, Grimes H Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. Nature, 2016-08-31;537(7622):698-702. 2016-08-31 [PMID: 27580035] (Flow Cytometry, Mouse)
• Lu T, Rothenberg M Bone Marrow Derived Eosinophil Cultures. Bio Protoc, 2014-06-20;4(12):. 2014-06-20 [PMID: 27540562] (Flow Cytometry, Mouse)
• Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1 Proc Natl Acad Sci USA, 2016-07-25;0(0):. 2016-07-25 [PMID: 27457925] (Flow Cytometry, Mouse)
• Schollaert , Kaila L, Stephens , Michael, Gray , Jerilyn, Fulkerson , Patricia Generation of eosinophils from cryopreserved murine bone marrow cells. PLoS ONE, 2014-12-31;9(12):e116141. 2014-12-31 [PMID: 25551463] (Flow Cytometry, Mouse)
• Takeda K, Shiraishi Y, Ashino S, Han J, Jia Y, Wang M, Lee N, Lee J, Gelfand E Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge. J Allergy Clin Immunol, 2014-10-11;135(2):451-60. 2014-10-11 [PMID: 25312762] (ICC, Mouse)
• Doyle , Alfred D, Jacobsen , Elizabet, Ochkur , Sergei I, Willetts , Lian, Shim , Kelly, Neely , Joseph, Kloeber , Jake, Lesuer , Will E, Pero , Ralph S, Lacy , Paige, Moqbel , Redwan, Lee , Nancy A, Lee , James J Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils. J Leukoc Biol, 2013-04-29;94(1):17-24. 2013-04-29 [PMID: 23630390] (Flow Cytometry, Mouse)
• Ge X, Ha S, Liu F, Rao S, Sriramarao P Eosinophil-expressed galectin-3 regulates cell trafficking and migration. Front Pharmacol, 2013-04-05;4(0):37. 2013-04-05 [PMID: 23576987] (Flow Cytometry, Mouse)
• Lu , Thomas X, Lim , Eun-Jin, Itskovich , Svetlana, Besse , John A, Plassard , Andrew J, Mingler , Melissa, Rothenberg , Joelle A, Fulkerson , Patricia, Aronow , Bruce J, Rothenberg , Marc E Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth. PLoS ONE, 2013-03-22;8(3):e59397. 2013-03-22 [PMID: 23533623] (Flow Cytometry, Mouse)
• Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation. J. Biol. Chem., 2011-09-12;286(44):38231-41. 2011-09-12 [PMID: 21911487] (ICC, Mouse)
• Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection. J. Immunol., 2011-07-29;187(5):2656-65. 2011-07-29 [PMID: 21804013] (Flow Cytometry, Mouse)
• Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M SIRP{alpha}/CD172a Regulates Eosinophil Homeostasis. J. Immunol., 2011-07-20;187(5):2268-77. 2011-07-20 [PMID: 21775684] (Flow Cytometry, Mouse)
• Cain DW, Snowden PB, Sempowski GD, Kelsoe G Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism. PLoS ONE, 2011-05-31;6(5):e19957. 2011-05-31 [PMID: 21655273] (Flow Cytometry, Mouse)
• Yamada T, Tani Y, Nakanishi H Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. FASEB J., 2010-10-19;25(2):561-8. 2010-10-19 [PMID: 20959515] (Flow Cytometry, Mouse)
• Shilling RA, Clay BS, Tesciuba AG, Berry EL, Lu T, Moore TV, Bandukwala HS, Tong J, Weinstock JV, Flavell RA, Horan T, Yoshinaga SK, Welcher AA, Cannon JL, Sperling AI CD28 and ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo. Cell. Immunol., 2009-07-10;259(2):177-84. 2009-07-10 [PMID: 19646680] (Flow Cytometry, Mouse)
• Cannon JL, Collins A, Mody PD, Balachandran D, Henriksen KJ, Smith CE, Tong J, Clay BS, Miller SD, Sperling AI CD43 regulates Th2 differentiation and inflammation. J. Immunol., 2008-06-01;180(11):7385-93. 2008-06-01 [PMID: 18490738] (Flow Cytometry, Mouse)
• Gupta S, Fuchs B, Schulz-Maronde S, Heitland A, Escher SE, Mack M, Tillmann HC, Braun A, Forssmann WG, Elsner J, Forssmann U Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation. J. Leukoc. Biol., 2007-12-06;83(3):765-73. 2007-12-06 [PMID: 18063698] (Flow Cytometry, Mouse)
• Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells. Allergy, 2007-12-01;62(12):1410-8. 2007-12-01 [PMID: 17983375] (Flow Cytometry, Mouse)
• Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma. J. Immunol., 2007-06-15;178(12):7879-89. 2007-06-15 [PMID: 17548626] (Flow Cytometry, Mouse)
• Fulkerson PC, Fischetti CA, McBride ML, Hassman LM, Hogan SP, Rothenberg ME A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc. Natl. Acad. Sci. U.S.A., 2006-10-23;103(44):16418-23. 2006-10-23 [PMID: 17060636] (Flow Cytometry, Mouse)
• Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F CD48 is an allergen and IL-3-induced activation molecule on eosinophils. J. Immunol., 2006-07-01;177(1):77-83. 2006-07-01 [PMID: 16785501] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR3
• Uniprot:
  • P51678()