CCR10/GPR2 Antibody (314305R) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human CCR10 and eGFP was stained with (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478R) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 314305R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCR10/GPR2 Antibody (314305R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Y3 rat myeloid cell line transfected with human CCR10
  Gln8-Asn362
  Accession # P46092
• Specificity: Detects human CCR10 transfectants but not the parental cell line in Flow Cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (314305R) [Unconjugated]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P46092()