CCL4/MIP-1 beta Antibody - BSA Free

Western Blot: CCL4L1/LAG-1 Antibody [NBP1-77897] - Shows detection of recombinant swine MIP-1b (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 (TM) conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.

• Reactivity: Po
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CCL4/MIP-1 beta Antibody - BSA Free Summary

• Description: This product was Protein-A purified from monospecific antiserum by chromatography
  
  Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein-A purified CCL4/MIP-1 beta Antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP-1 beta protein. (Uniprot: Q711P4)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCL4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Western Blot 1:500
• Application Notes: This protein-A purified This product has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with CCL4/MIP-1 beta from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4/MIP-1 beta based on 90% homology. Cross-reactivity with CCL4/MIP-1 beta from other sources has not been determined. The swine CCL4/MIP-1 beta sequence is also 86% homologous to swine CCL3L1.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for CCL4/MIP-1 beta Antibody - BSA Free

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP-1 beta/CCL4
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL4
• Uniprot:
  • Q711P4(Porcine)