CCL4/MIP-1 beta Antibody

Product Discontinued

CCL4/MIP-1 beta Antibody Summary

• Immunogen: Purified insect cell line Sf21-derived recombinant human MIP-1beta.
• Localization: Secreted
• Specificity: Macrophage Inflammatory Protein 1 beta. NB120-10383 will neutralize the biological activity of recombinant human, but not mouse MIP-1beta. It will not neutralize the biological activity of recombinant human or mouse MIP-1alpha.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
• Application Notes: Neutralization Dose (ND50) is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is approximately 0.5 - 2 ug/ml in the presence of 0.1 ug/ml of human MIP-1beta, using the human monocyte chemotaxis assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CCL4/MIP-1 beta Antibody

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP-1 beta/CCL4
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD-5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL4
• Entrez:
  • 6351(Human)