Recombinant Human CCL23/Ck beta 8-1/MIP3 Protein

SDS-Page: Human CCL23/Ck beta 8-1/MIP3 Protein [NBP2-35021]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CCL23/Ck beta 8-1/MIP3 Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 99 amino acids corresponding to CCL23/Ck beta 8-1/MIP3 Source: E. coli

  Uniprot ID: P55773

  Amino Acid Sequence: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN

• Details of Functionality: Macrophage Inflammatory Protein 3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL23
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL23/Ck beta 8-1/MIP3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CCL23/Ck beta 8-1/MIP3 Protein

• C6 beta-chemokine
• CCL23/Ck beta 8-1
• chemokine (C-C motif) ligand 23
• Ck beta 8-1
• CKb8
• Ckb-8
• Ckb81
• CK-beta-8
• hmrp-2a
• Macrophage inflammatory protein 3
• MIP3
• MIP-3
• MIP-3/CCL23
• MIP-3CK-BETA-8
• MPIF1
• MPIF-1Ckb-8-1
• Myeloid progenitor inhibitory factor 1
• SCYA23C-C motif chemokine 23
• small inducible cytokine subfamily A (Cys-Cys), member 23
• Small-inducible cytokine A23

Background

CCL23 is a member of CC chemokine family and is encoded by CCL23 gene located on Chr.17 in humans, where near several other CC chemokines. Highly expressed in adult lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30- 99).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL23
• Uniprot:
  • P55773()
  • P55773()
  • P55773()