CCL21/6Ckine Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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CCL21/6Ckine Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL21/6Ckine (R&D Systems, Catalog # 366-6C)
Ser24-Pro134
Accession # Q6ICR7
Specificity
Detects human CCL21/6Ckine in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) TARC, rhMIP‑1δ, rhEotaxin‑2, rhTECK, and recombinant mouse 6Ckine is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL21
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human CCL21/6Ckine Antibody (Catalog # MAB3661)
ELISA Detection: Human CCL21/6Ckine Biotinylated Antibody (Catalog # BAF366)
Standard: Recombinant Human CCL21/6Ckine (Catalog # 366-6C)
Readout System
Publications
Read Publications using
BAF366 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL21/6Ckine Antibody [Biotin]

  • 6Ckine
  • 6CkineSmall-inducible cytokine A21
  • Beta-chemokine exodus-2
  • CCL21
  • chemokine (C-C motif) ligand 21
  • CKb9
  • exodus-2
  • member 21
  • SCYA21
  • SCYA21MGC34555
  • secondary lymphoid tissue chemokine
  • SLC
  • SLCSecondary lymphoid-tissue chemokine
  • TCA-4

Background

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus-2, shows 21 - 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for MIP‑3 beta /ELC, another novel CC chemokine.

Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines so far tested.

  1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL21/6Ckine Antibody (BAF366)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, IHC-P.


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(1)
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Secondary Antibodies

 

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Biotin BAF366

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Bioinformatics

Gene Symbol CCL21
Uniprot