CCL2/JE/MCP-1 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL2/JE/MCP-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)
  Gln24-Arg96
  Accession # Q5SVU3
• Specificity: Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CCL2/JE/MCP-1 Antibody (Catalog # AF-479-NA)
  ELISA Detection: Mouse CCL2/JE/MCP-1 Biotinylated Antibody (Catalog # BAF479)
  Standard: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody [Biotin]

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
3. Luini, W. et al. (1994) Cytokine 6:28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/JE/MCP-1 Antibody (BAF479)(17)

Publications using BAF479

• Hong, J;Son, M;Sin, J;Kim, H;Chung, DK; Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation Nutrients 2023-11-09 [PMID: 38004123] (ELISA Detection, Mouse)
• Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity, 2015-11-17;43(5):945-58. 2015-11-17 [PMID: 26588780] (ELISA Development (Detection), Mouse)
• M. E. Mikucki, D. T. Fisher, J. Matsuzaki, J. J. Skitzki, N. B. Gaulin, J. B. Muhitch et al. Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints Nature Communications 2015-06-25 [PMID: 26109379]
• Meng Y, Efimova EV, Hamzeh KW Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine. Mol. Ther., 2012-02-14;20(5):1046-55. 2012-02-14 [PMID: 22334019] (IHC-P, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells. PLoS ONE, 2011-07-25;6(7):e22504. 2011-07-25 [PMID: 21799877] (ELISA Development, Mouse)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse)
• Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. J. Immunol., 2006-07-01;177(1):612-20. 2006-07-01 [PMID: 16785559] (ELISA Development, Mouse)
• Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Blood, 2005-11-03;107(5):2112-22. 2005-11-03 [PMID: 16269622] (IHC, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse)
• Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA Development, Human)
• Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas. Immunol. Lett., 2002-06-03;82(1):111-21. 2002-06-03 [PMID: 12008042] (ELISA Development, Mouse)
• Janatpour MJ, Hudak S, Sathe M, McEvoy LM Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment. J. Exp. Med., 2001-11-05;194(9):1375-84. 2001-11-05 [PMID: 11696601] (IHC, Mouse)
• Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. J. Immunol., 2000-03-01;164(5):2650-9. 2000-03-01 [PMID: 10679105] (ELISA Development, Rat)

Review for CCL2/JE/MCP-1 Antibody (BAF479) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 01/15/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID: 23410977
• Species: Mouse

Bioinformatics

• Gene Symbol: Ccl2
• Uniprot:
  • Q5SVU3()