CCL2/JE/MCP-1 Products

479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">

479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox" target="_blank">

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox" target="_blank">

MAB679) at 20ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and membrane. " class="big_lightbox" target="_blank">

279-MC) was serially diluted and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND₅₀ is typically 0.9-4.5 µg/mL." class="big_lightbox" target="_blank">