CCL19/MIP-3 beta Antibody (87102) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap), ELISA(Det)
• Clone: 87102
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

CCL19/MIP-3 beta Antibody (87102) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse CCL19/MIP-3β
  Gly26-Val107-Leu-Glu (Ser108LeuGlu)
  Accession # Q548P0
• Specificity: Detects mouse CCL19/MIP‑3 beta in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody (87102) [Alexa Fluor® 647]

• beta chemokine exodus-3
• Beta-chemokine exodus-3
• CC chemokine ligand 19
• C-C motif chemokine 19
• CCL19
• chemokine (C-C motif) ligand 19
• CKb11
• EBI1-ligand chemokine
• ELC
• ELCMIP-3-beta
• Epstein-Barr virus-induced molecule 1 ligand chemokine
• exodus-3
• Macrophage inflammatory protein 3 beta
• macrophage inflammatory protein 3-beta
• MGC34433
• MIP3 beta
• MIP-3 beta
• MIP-3b
• MIP3BCK beta-11
• SCYA19EBI1 ligand chemokine
• small inducible cytokine subfamily A (Cys-Cys), member 19
• Small-inducible cytokine A19

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a beta  chemokine that binds specifically to the chemokine receptor CCR7/EBI-1/BLR-2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant CCL19 has been shown to be chemotactic for T-cells and B-cells. The CCL19 receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up‑regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.