Recombinant Rhesus Macaque CCL17/TARC Protein

• Reactivity: RM
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Rhesus Macaque CCL17/TARC Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 71 amino acids corresponding to CCL17/TARC Source: E. coli

  Uniprot ID: Q8HYP9

  Amino Acid Sequence: ARGTNVGREC CLKYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQNKAIC SDPNDKKVKK ALKYLQSLER S

• Details of Functionality: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL17
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CCL17/TARC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 7.4, with 0.02 % Tween-20.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rhesus Macaque CCL17/TARC Protein

• ABCD-2
• CC chemokine TARC
• C-C motif chemokine 17
• CCL17
• chemokine (C-C motif) ligand 17
• MGC138273
• SCYA17
• SCYA17MGC138271
• small inducible cytokine subfamily A (Cys-Cys), member 17
• Small-inducible cytokine A17
• T cell-directed CC chemokine
• TARC
• TARCA-152E5.3
• Thymus and activation-regulated chemokine

Background

TARC is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL17