CCL17/TARC Antibody (110904)

Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.

CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant rat:mouse chimeric antibody having a mouse IgG1 Fc or (D) MAB529 (rat antibody) which is a commercial antibody that neutralizes CCL17. Assay was repeated twice with five replicates per sample each time. Data are expressed as chemotactic index (CI = Average RLU from stimulated wells/average RLU from medium control; medium control = CI of 1). Antibody dose used for each sample is indicated along the x axis. Composite CI results from both experiments are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24339934), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 110904
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL17/TARC Antibody (110904) Summary

• Immunogen: E. coli-derived Recombinant Mouse CCL17/TARC
  Ala24-Pro93
  Accession # Q9WUZ6
• Specificity: Detects mouse CCL17/TARC in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.75-3.0 ug/mL
• Application Notes: ELISA Detection: Mouse CCL17/TARC Biotinylated Antibody (Catalog number BAF529)
  Standard: Recombinant Mouse CCL17/TARC (Catalog number 529-TR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL17/TARC Antibody (110904)

• ABCD-2
• CC chemokine TARC
• C-C motif chemokine 17
• CCL17
• chemokine (C-C motif) ligand 17
• MGC138273
• SCYA17
• SCYA17MGC138271
• small inducible cytokine subfamily A (Cys-Cys), member 17
• Small-inducible cytokine A17
• T cell-directed CC chemokine
• TARC
• TARCA-152E5.3
• Thymus and activation-regulated chemokine

Background

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha , MIP-1 beta , MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
3. Nomiyama, H. et al. (1997) Genomics 40:211.
4. Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL17/TARC Antibody (MAB529)(19)

Publications using MAB529

• Yang Zhang, Yicong Ye, Xiaoqiang Tang, Hui Wang, Toshiko Tanaka, Ran Tian et al. CCL17 acts as a novel therapeutic target in pathological cardiac hypertrophy and heart failure Journal of Experimental Medicine 8/1/2022 [PMID: 35687056]
• S Deng, P Jin, P Sherchan, S Liu, Y Cui, L Huang, JH Zhang, Y Gong, J Tang Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice Journal of Neuroinflammation, 2021-03-01;18(1):62. 2021-03-01 [PMID: 33648537] (Neutralization, Mouse)
• MC Lee, R Saleh, A Achuthan, AJ Fleetwood, I Förster, JA Hamilton, AD Cook CCL17 blockade as a therapy for osteoarthritis pain and disease Arthritis Res. Ther., 2018-04-05;20(1):62. 2018-04-05 [PMID: 29622035] (Neutralization, Mouse)
• AD Cook, MC Lee, R Saleh, HW Khiew, AD Christense, A Achuthan, AJ Fleetwood, DC Lacey, JE Smith, I Förster, JA Hamilton TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17 JCI Insight, 2018-03-22;3(6):. 2018-03-22 [PMID: 29563337] (Neutralization, Mouse)
• L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo Mol. Ther., 2017-10-13;0(0):. 2017-10-13 [PMID: 29103909] (Neutralization, Mouse)
• iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation Cell Rep, 2016-09-20;16(12):3260-72. 2016-09-20 [PMID: 27653688] (Neutralization, Mouse)
• Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation J Clin Invest, 2016-08-15;0(0):. 2016-08-15 [PMID: 27525438] (Neutralization, Mouse)
• Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. Am J Pathol, 2014-12-06;185(2):420-31. 2014-12-06 [PMID: 25485859] (Cell Selection, Mouse)
• Santulli-Marotto S, Boakye K, Lacy E, Wu S, Luongo J, Kavalkovich K, Coelho A, Hogaboam C, Ryan M Engagement of two distinct binding domains on CCL17 is required for signaling through CCR4 and establishment of localized inflammatory conditions in the lung. PLoS ONE, 2013-12-05;8(12):e81465. 2013-12-05 [PMID: 24339934] (Direct ELISA, Neutralization, Mouse)
• Sandra Santulli-Marotto, Jamie Fisher, Theodore Petley, Ken Boakye, Tadas Panavas, Jennifer Luongo et al. Surrogate Antibodies That Specifically Bind and Neutralize CCL17 But Not CCL22 Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 6/12/2013 [PMID: 23750473]
• Sandra Santulli-Marotto, Jamie Fisher, Theodore Petley, Ken Boakye, Tadas Panavas, Jennifer Luongo et al. Surrogate Antibodies That Specifically Bind and Neutralize CCL17 But Not CCL22 Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2013-06-12 [PMID: 23750473] (Neutralization, E. coli)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Specht S, Frank JK, Alferink J, Dubben B, Layland LE, Denece G, Bain O, Forster I, Kirschning CJ, Martin C, Hoerauf A CCL17 controls mast cells for the defense against filarial larval entry. J. Immunol., 2011-03-11;186(8):4845-52. 2011-03-11 [PMID: 21398605] (Neutralization, Mouse)
• Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA Development, Mouse)
• Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17. J. Immunol., 2008-12-15;181(12):8576-84. 2008-12-15 [PMID: 19050277] (Neutralization, Mouse)
• Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA Development, Mouse)
• Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity. J. Immunol., 2007-10-01;179(7):4661-71. 2007-10-01 [PMID: 17878364] (Neutralization, Mouse)
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Neutralization, Mouse)
• Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL17
• Uniprot:
  • Q9WUZ6()