CCL16/HCC-4/LEC Antibody (70218) [Unconjugated]

Images

 
Recombinant Human CCL16/HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
70218
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CCL16/HCC-4/LEC Antibody (70218) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL16/HCC-4
Gln24-Gln120
Accession # O15467
Specificity
Detects human CCL16/HCC‑4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL16
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 5-20 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CCL16/HCC-4 Biotinylated Affinity Purified PAb (Catalog number BAF802)
Standard: Recombinant Human CCL16/HCC-4 (Catalog number 802-HC)
Publications
Read Publication using
MAB328 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL16/HCC-4/LEC Antibody (70218) [Unconjugated]

  • C-C motif chemokine 16
  • CCL16
  • chemokine (C-C motif) ligand 16
  • Chemokine CC-4
  • Chemokine LEC
  • CKb12
  • HCC4
  • HCC-4
  • HCC-4Liver-expressed chemokine
  • LCC-1Lymphocyte and monocyte chemoattractant
  • LEC
  • liver CC chemokine-1
  • LMC
  • LMCSmall-inducible cytokine A16
  • monotactin-1
  • Mtn-1
  • NCC-4
  • NCC4IL-10-inducible chemokine
  • NCC-4ILINCK
  • new CC chemokine 4
  • SCYA16MGC117051
  • SCYL4
  • small inducible cytokine subfamily A (Cys-Cys), member 16

Background

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.

Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

  1. Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
  2. Hedrick, J. et al. (1998) Blood 91:4242.
  3. Youn, B-S. et al. (1998) BBRC 247:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL16/HCC-4/LEC Antibody (MAB328)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Neut.


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Bioinformatics

Gene Symbol CCL16
Uniprot