Recombinant Human CCL15/MIP-1 delta Protein

SDS-Page: Human CCL15/MIP-1 delta Protein [NBP2-35065]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CCL15/MIP-1 delta Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 92 amino acids corresponding to CCL15/MIP-1 delta Source: E. coli

  Uniprot ID: Q16663

  Amino Acid Sequence: QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI

• Details of Functionality: Macrophage Inflammatory Protein 5 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL15
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL15/MIP-1 delta as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 10.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CCL15/MIP-1 delta Protein

• CCL15
• chemokine (C-C motif) ligand 15
• Chemokine CC-2
• HCC-2
• HCC-2Lkn-1
• Leukotactin 1
• Leukotactin-1
• LKN1
• LKN-1
• Macrophage inflammatory protein 5
• MIP1 delta
• MIP-1 delta
• MIP5
• MIP-5
• MIP-5/CCL15
• MIP-5MIP-1d
• Mrp-2b
• NCC-3
• NCC-3MIP-1D
• NCC3MRP-2B
• new CC chemokine 3
• SCYA15member 15
• Small-inducible cytokine A15

Background

Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. CCL15 has several cleaved chains. All of them are more potent chemoattractants than CCL15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL15
• Uniprot:
  • Q16663()
  • Q16663()
  • Q16663()