CCL15/MIP-1 delta Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL15/MIP-1 delta Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL15/MIP-1δ (R&D Systems, Catalog #628-LK)
  Ser46-Ile113
  Accession # Q16663
• Specificity: Detects human CCL15/MIP-1δ in Western blots. In this format, this antibody shows a preference for the 68 amino acid (aa) residue isoform over the 92 aa isoform of human CCL15/MIP-1δ and less than 1% cross-reactivity with rh6Ckine, rhBLC/BCA-1, rhENA-78, rhEotaxin, rhEotaxin-2, rhFractalkine, rhGCP‑2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhHCC‑1, rhHCC‑4, rhI-309, rhIL-8, rhMCP‑1, rhMCP-2, rhMCP‑3, rhMCP‑4, rhMDC, rhMIG, rhMIP‑1 alpha , rhMIP‑1 beta , rhMIP‑3 alpha , rhMIP-3 beta , rhMPIF‑1, rhNAP‑2, rhPARC, rhRANTES, rhSDF‑1 alpha , rhSDF‑1 beta , rhTarc, rhTECK, and rhVIC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL15
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL15/MIP-1 delta Antibody [Biotin]

• CCL15
• chemokine (C-C motif) ligand 15
• Chemokine CC-2
• HCC-2
• HCC-2Lkn-1
• Leukotactin 1
• Leukotactin-1
• LKN1
• LKN-1
• Macrophage inflammatory protein 5
• MIP1 delta
• MIP-1 delta
• MIP5
• MIP-5
• MIP-5MIP-1d
• Mrp-2b
• NCC-3
• NCC-3MIP-1D
• NCC3MRP-2B
• new CC chemokine 3
• SCYA15member 15
• Small-inducible cytokine A15

Background

CCL15, also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. CCL15/LKN-1, together with mouse C10, mouse MIP-1 gamma  and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in CCL15/LKN-1 have been shown to form a third disulfide bond CCL15/LKN-1 cDNA encodes a 113 amino acid (aa) residue precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 aa residue mature protein. In recombinant CCL15/LKN-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of CCL15/LKN-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 aa residues, respectively. The full length and the amino-terminal truncated forms of human CCL15δ/LKN-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR1 transfected cells. Additionally, CCL15/LKN-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors.

1. Youn, B.-S. et al. (1997) J. Immunol. 159:5201.
2. Pardigol, A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6308.
3. Wang, W. et al. (1998) J. Clinical Immunol.18:214.
4. Coulin, F. et al. (1997) Eur. J. Biochem. 248:507.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL15
• Uniprot:
  • Q16663()