CCL11/Eotaxin Antibody (43911) [Unconjugated]

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND₅₀ is typically 1-5 µg/mL." > 320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND₅₀ is typically 1-5 µg/mL." title="Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND₅₀ is typically 1-5 µg/mL." />

Recombinant Human CCL11/Eotaxin (Catalog # <a class=

MAB320) at 20ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. " > MAB320) at 20ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. " title="Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Eotaxin mRNA (ACD RNAScope Probe, catalog # 438468; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human Eotaxin monoclonal antibody (R&D Systems catalog # MAB320) at 20ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. " />

Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Eotaxin mRNA (ACD RNAScope Probe, catalog # 438468; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human Eotaxin monoclonal antibody (R&D Systems catalog # <a class=

320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human CCL11/Eotaxin (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC, B/N, Dual ISH-IHC, ELISA(Cap)
• Clone: 43911
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL11/Eotaxin Antibody (43911) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL11/Eotaxin
  Gly24-Pro97
  Accession # P51671
• Specificity: Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 14, 17, 19, 20, 21, 25, recombinant mouse CCL2, 3 , 4 , 5 ,6 , 7, 9/10/MIP-1 gamma , 11, 21, or 25.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 3-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 1-5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL11/Eotaxin Biotinylated Antibody (Catalog number BAF320)
  Standard: Recombinant Human CCL11/Eotaxin (Catalog number 320-EO)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL11/Eotaxin Antibody (43911) [Unconjugated]

• C-C motif chemokine 11
• CCL11
• chemokine (C-C motif) ligand 11
• Eosinophil chemotactic protein
• Eotaxin
• eotaxin-1
• MGC22554
• SCYA11Small-inducible cytokine A11
• small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
4. Choe, H. et al. (1996) Cell 85:1135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL11/Eotaxin Antibody (MAB320)(10)

Publications using MAB320

• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human)
• Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Nature, 2011-08-31;477(7362):90-4. 2011-08-31 [PMID: 21886162] (Neutralization, Mouse)
• Miyagaki T, Sugaya M, Fujita H Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma. J. Invest. Dermatol., 2010-05-27;130(9):2304-11. 2010-05-27 [PMID: 20505746] (IHC-P, Human)
• Lena Uller, Cecilia Ahlström Ahlström Emanuelsson, Morgan Andersson, Jonas S Erjefält, Lennart Greiff, Carl G Persson Early phase resolution of mucosal eosinophilic inflammation in allergic rhinitis Respiratory Research 12/1/2010 [PMID: 20459697]
• Osei-Kumah A, Smith R, Clifton VL Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Cytokine, 2008-07-17;43(2):187-93. 2008-07-17 [PMID: 18639465] (ELISA Development, Human)
• Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema. J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25. 2007-11-01 [PMID: 17983873] (IHC-P, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA Development, Neutralization, Human)
• Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts. J. Allergy Clin. Immunol., 2005-12-02;117(1):103-10. 2005-12-02 [PMID: 16387592] (Neutralization, Human)
• Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission. Parasitology, 2005-03-01;130(0):333-42. 2005-03-01 [PMID: 15796016] (ELISA Development, Human)
• Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatr. Res., 2000-05-01;47(5):592-7. 2000-05-01 [PMID: 10813582] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL11
• Uniprot:
  • P51671()