CCL1/I-309/TCA-3 Antibody (148113)

Recombinant Mouse CCL1/TCA‑3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA‑3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 148113
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL1/I-309/TCA-3 Antibody (148113) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3
  Lys24-Cys92
  Accession # P10146
• Specificity: Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5 ,6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 2-10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL1/I-309/TCA-3 Antibody (148113)

• C-C motif chemokine 1
• CCL1
• chemokine (C-C motif) ligand 1
• I-309
• inflammatory cytokine I-309
• P500
• SCYA1
• SCYA1Small-inducible cytokine A1
• SCYA2
• SISe
• small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• T lymphocyte-secreted protein I-309
• TCA3
• TCA-3

Background

Mouse CCL1, also known as TCA-3, is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha , MIP-1 beta and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.

1. Goya, I. et al. (1998) J. Immunol. 160:1975.
2. Burd, P. et al. (1987) J. Immunol. 139:3126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL1/I-309/TCA-3 Antibody (MAB845)(8)

Publications using MAB845

• Chen, L;Zou, X;Liu, CC;Yan, P;Deng, J;Wang, C;Chen, MY;Tang, XQ;Shi, JM;Xin, WJ;Zhang, XZ;Feng, X;Xu, T;Xie, JD; Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1-mediated accumulation of perivascular macrophages Science advances 2025-04-18 [PMID: 40238872] (In vivo assay, Mouse)
• Xie, B;Wang, M;Zhang, X;Zhang, Y;Qi, H;Liu, H;Wu, Y;Wen, X;Chen, X;Han, M;Xu, D;Sun, X;Zhang, X;Zhao, X;Shang, Y;Yuan, S;Zhang, J; Gut-derived memory ?? T17 cells exacerbate sepsis-induced acute lung injury in mice Nature communications 2024-08-07 [PMID: 39112475] (Western Blot, In vivo assay, Mouse)
• Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al. Cytotoxic T cells swarm by homotypic chemokine signalling eLife 2020-10-13 [PMID: 33046212]
• L Li, L Ni, RF Heary, S Elkabes Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation, 2020-02-25;17(1):73. 2020-02-25 [PMID: 32098620] (Neutralization, Mouse)
• Katarina Radulovic, Valerio Rossini, Calin Manta, Karlheinz Holzmann, Hans A. Kestler, Jan Hendrik Niess The Early Activation Marker CD69 Regulates the Expression of Chemokines and CD4 T Cell Accumulation in Intestine PLoS ONE 2013-06-12 [PMID: 23776480]
• Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011-01-09;12(2):167-77. 2011-01-09 [PMID: 21217759] (Neutralization, Mouse)
• Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA Development, Mouse)
• Mosmann T Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Eur. J. Immunol., 2004-06-01;34(6):1617-26. 2004-06-01 [PMID: 15162431] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL1
• Uniprot:
  • P10146()