Ccd1/DIXDC1 Antibody - BSA Free

Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:77 kDa

Immunocytochemistry/Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP3-12691] - Staining of DIXDC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DIXDC1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-DIXDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-DIXDC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 77 kDaObserved band size :95 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ccd1/DIXDC1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Ccd1/DIXDC1 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIXDC1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Ccd1/DIXDC1 Antibody - BSA Free

• Ccd1
• Coiled-coil protein DIX1
• Coiled-coil-DIX1
• DIX domain containing 1
• DIX domain-containing protein 1
• Dixdc1
• Dixin
• Kiaa1735
• KIAA1735coiled-coil-DIX1

Background

DIXDC1 (DIX domain containing 1/Dixin) is a 683 amino acid protein. It positively regulates the Wnt signaling pathway by activating WNT3A via DVL2. It is expressed ubiquitously but has higher expression in skeletal and cardiac muscle. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. DIXDC1 may play a role in breast cancer, breast carcinoma, endometrial cancer, epithelial neoplasia, schizophrenia, colon cancer, and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIXDC1