MEKK4 Antibody - BSA Free

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: MEKK4 Antibody [NBP3-35705] - Western blot analysis of various lysates using MEKK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human placenta using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MEKK4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MEKK4 Antibody - BSA Free (NBP3-35705) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK4 (NP_005913.2).
  
  Sequence:
  MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 182 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MEKK4 Antibody - BSA Free

• EC 2.7.11.25
• FLJ42439
• MAPKKK4yeast, homolog of
• MEKK 4
• mitogen-activated protein kinase kinase kinase 4

Background

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K4