CC Chemokine Receptor D6 Overexpression Lysate (Native)

Images

 
Western Blot: Chemokine Receptor D6 Overexpression Lysate (Adult Normal) [NBL1-08759] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chemokine Receptor D6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CC Chemokine Receptor D6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202459 and based on accession number NM_001296. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chemokine binding protein 2 (CCBP2), mRNA.
Gene
CCBP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CC Chemokine Receptor D6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CC Chemokine Receptor D6 Overexpression Lysate (Native)

  • ACKR2
  • C-C chemokine receptor D6
  • CCBP2
  • CCR10CCR9D6CC-chemokine-binding receptor JAB61
  • chemokine (C-C) receptor 9
  • chemokine binding protein 2
  • Chemokine receptor CCR-10
  • Chemokine receptor CCR-9
  • chemokine receptor D6
  • chemokine-binding protein 2
  • Chemokine-binding protein D6
  • CMKBR9
  • CMKBR9chemokine (C-C motif) receptor 9
  • D6
  • hD6
  • MGC126678
  • MGC138250

Background

This gene encodes a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CC Chemokine Receptor D6 Lysate (NBL1-08759) (0)

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Bioinformatics

Gene Symbol CCBP2
Entrez