CBX3 Overexpression Lysate (Native)

Images

 
Western Blot: CBX3 Overexpression Lysate (Adult Normal) [NBL1-08749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CBX3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CBX3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201478 and based on accession number NM_007276. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromobox homolog 3 (HP1 gamma homolog, Drosophila) (CBX3), transcript variant 1, mRNA.
Gene
CBX3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CBX3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CBX3 Overexpression Lysate (Native)

  • chromobox homolog 3 (Drosophila HP1 gamma)
  • chromobox homolog 3
  • chromobox protein homolog 3
  • Drosophila)
  • HECH
  • Heterochromatin protein 1 homolog gamma
  • heterochromatin protein HP1 gamma
  • heterochromatin-like protein 1
  • HP1 gamma homolog
  • HP1 gamma
  • HP1-GAMMA
  • Modifier 2 protein

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) contains an alternate 5' exon but encodes the same protein as transcript variant 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CBX3 Lysate (NBL1-08749) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CBX3