CBP/KAT3A Antibody (HL2197) - Azide and BSA Free

Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2197
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CBP/KAT3A Antibody (HL2197) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CBP/KAT3A Antibody (HL2197) - Azide and BSA Free (NBP3-25399) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragmemt of human CBP/KAT3A
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CREBBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot Assay dependent
• Theoretical MW: 265 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CBP/KAT3A Antibody (HL2197) - Azide and BSA Free

• CBP
• CBPRTS
• CREB binding protein
• CREB-binding protein
• CREBBP
• EC 2.3.1.48
• KAT3A
• RSTS
• RTS
• Rubinstein-Taybi syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CREBBP