CBARA1 Antibody

Images

 
Western Blot: CBARA1 Antibody [NBP1-86663] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human liver shows moderate granular positivity in cytoplasm in hepatocytes.
Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human endometrium shows strong granular positivity in cytoplasm in glandular cells.
Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human kidney shows strong granular positivity in cytoplasm in cells in tubules.
Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human rectum shows strong granular positivity in cytoplasm in glandular cells.
Simple Western: CBARA1 Antibody [NBP1-86663] - Simple Western lane view shows a specific band for CBARA1 in 0.2 mg/ml of RT-4 (left), U-251 (right) lysate. This experiment was performed under reducing conditions using ...read more
Simple Western: CBARA1 Antibody [NBP1-86663] - Electropherogram image(s) of corresponding Simple Western lane view. CBARA1 antibody was used at 1:20 dilution on RT-4 and U-251 lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CBARA1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFL
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MICU1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Use in WB reported in scientific literature (PMID 28292968).
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CBARA1 Protein (NBP1-86663PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-86663 in the following applications:

Publications
Read Publications using
NBP1-86663 in the following applications:

  • 2 publications
  • WB
    5 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24336167). Use in Mouse reported in scientific literature (PMID:32075766).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CBARA1 Antibody

  • allergen Hom s 4
  • ara CALC
  • Atopy-related autoantigen CALC
  • CALC
  • CALCDKFZp564C246
  • calcium binding atopy-related autoantigen 1
  • Calcium-binding atopy-related autoantigen 1
  • CBARA1
  • MICU1
  • MICU1mitochondrial
  • mitochondrial calcium uptake 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-86663
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P

Publications for CBARA1 Antibody (NBP1-86663)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(2)
WB
(5)
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(3)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using NBP1-86663 Applications Species
Di Marco G, Vallese F, Jourde B et al. A High-Throughput Screening Identifies MICU1 Targeting Compounds Cell Rep Feb 18 2020 [PMID: 32075766] (WB, Mouse) WB Mouse
Ji L, Liu F, Jing Z et al. MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response. Diabetes. Mar 14 2017 [PMID: 28292968] (WB, IHC-P) WB, IHC-P
Lee Y, Min CK, Kim TG et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep 2015 Oct [PMID: 26341627] (WB, Human) WB Human
Wang W, Xie Q, Zhou X et al. Mitofusin-2 triggers mitochondria Ca(2+) influx from the endoplasmic reticulum to induce apoptosis in hepatocellular carcinoma cells. Cancer Lett. 2014 Dec 23 [PMID: 25541060] (WB, IHC-P, Human)

Details:
CBARA1 (MICU1) antibody used for immunohistochemisty on paraffin-embedded HCC and adjacent normal tissues to compare the expression of CBARA1 (MICU1). Immunohistochemistry analysis showing no significant difference between HCC and adjacent normal tissues. Additionally, western blot analysis showing low protein expression levels of MICU1 (Fig. 11).
WB, IHC-P Human
Stephanie S Lam, Jeffrey D Martell, Kimberli J Kamer et al. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nature Methods 2014 Nov 24 [PMID: 25419960] (WB, Human) WB Human
Logan CV, Szabadkai G, Sharpe JA et al. Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nat Genet 2014 Feb [PMID: 24336167]

Review for CBARA1 Antibody (NBP1-86663) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-86663:
Filter by Applications
Western Blot
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CBARA1 NBP1-86663
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 06/11/2014
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Summary

ApplicationWestern Blot
Sample TestedHUMAN CANCER CELL LINES, WHOLE CELL LYSATES
SpeciesHuman
LotB90039

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Video Protocols

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
06/11/2014
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol MICU1
Entrez
Uniprot