Cav3.2 Antibody (S55/10)

Images

 
Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken ...read more
Immunohistochemistry-Paraffin: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel ...read more
Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260 kDa.
Immunohistochemistry-Frozen: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
Clone
S55/10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Cav3.2 Antibody (S55/10) Summary

Immunogen
Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2
Localization
Membrane
Specificity
Detects approx 260 kDa. No cross-reactivity against Cav1.3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CACNA1H
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
Application Notes
1 ug/ml of Cav3.2 Antibody was sufficient for detection of Cav3.2 in 10 ug of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-22444 in the following applications:

Publications
Read Publications using
NBP1-22444 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Cav3.2 Antibody (S55/10)

  • CACNA1HB
  • calcium channel, voltage-dependent, T type, alpha 1H subunit
  • calcium channel, voltage-dependent, T type, alpha 1Hb subunit
  • Cav3.2
  • ECA6
  • EIG6
  • FLJ90484
  • Low-voltage-activated calcium channel alpha1 3.2 subunit
  • low-voltage-activated calcium channel alpha13.2 subunit
  • voltage dependent t-type calcium channel alpha-1H subunit
  • voltage-dependent T-type calcium channel subunit alpha-1H
  • voltage-gated calcium channel alpha subunit Cav3.2
  • voltage-gated calcium channel alpha subunit CavT.2
  • Voltage-gated calcium channel subunit alpha Cav3.2

Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (3, 4). Studies also suggest that the up-regulations of Cav3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cav3.2 Antibody (NBP1-22444)(9)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(7)
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Mouse
(5)
Rat
(1)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-22444 Applications Species
Zhang Y, Qian Z, Jiang D Et al. Neuromedin B receptor stimulation of Cav3.2 T-type Ca2+ channels in primary sensory neurons mediates peripheral pain hypersensitivity Theranostics Sep 9 2021 [PMID: 34646374] (WB, Mouse) WB Mouse
Gandini MA, Souza IA, Khullar A Et al. Regulation of CaV3.2 channels by the receptor for activated C kinase 1 (Rack-1) Pflugers Archiv : European journal of physiology Oct 8 2021 [PMID: 34623515] (WB) WB
Fan G, Kamann M, Cui Y et Al. Age attenuates the T-type CaV 3.2-RyR axis in vascular smooth muscle Aging Cell Mar 18 2020 [PMID: 32187825] (WB, Mouse) WB Mouse
Wang H, Wei Y, Pu Y et al. Brain-derived neurotrophic factor stimulation of T-type Ca2+ channels in sensory neurons contributes to increased peripheral pain sensitivity Sci Signal Sep 24 2019 [PMID: 31551295] (WB, Rat) WB Rat
Garcia-Caballero A, Gandini MA, Huang S et al. Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC Mol Brain Feb 8 2019 [PMID: 30736831] (WB, Mouse) WB Mouse
Hashad AM, Harraz OF, Brett SE. Caveolae Link CaV3.2 Channels to BKCa-Mediated Feedback in Vascular Smooth Muscle Vascular Biology [PMID: 30354206] (WB) WB
Garcia-Caballero A, Zhang FX, Hodgkinson V et al. T-type calcium channels functionally interact with spectrin (a/B) and ankyrin B Mol Brain May 2 2018 [PMID: 29720258] (ICC/IF) ICC/IF
Stemkowski P, Garcia-Caballero A, Gadotti VM et al. TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity. Cell Rep. Dec 13 2016 [PMID: 27974205] (Mouse) Mouse
Garca-Caballero A, Gadotti VM, Stemkowski P et al. The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity. Neuron. 2014 Sep 03 [PMID: 25189210] (WB, IP, Mouse) WB, IP Mouse

Review for Cav3.2 Antibody (NBP1-22444) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-22444:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Other 05/13/2014
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Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesOther
Lot0907

Blocking

Blocking Details5% milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 dilution at 4oC overnight in 1% BSA in PBST

Secondary Antibody

Secondary DescriptionGoat anti mouse IgG, HRP
Secondary Manufacturer Cat#Millipore AP308P
Secondary Concentration1:2000 dilution

Details

Detection NotesECL prime for 1 second (I should have use ECL).

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cav3.2 Antibody (NBP1-22444) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
05/13/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CACNA1H
Entrez
Uniprot