Cathepsin S Antibody (248718)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 248718
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin S Antibody (248718) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
  Gln17-Ile331 (predicted)
  Accession # P25774
• Specificity: Detects pro and mature forms of human Cathepsin S in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, D, L, L2/V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSS
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin S Antibody (248718)

• Cathepsin S
• CTSS
• EC 3.4.22
• EC 3.4.22.27
• FLJ50259
• MGC3886

Background

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
2. Turk, V. et al. (2001) EMBO J. 20:4629.
3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin S Antibody (MAB1183)(1)

Publication using MAB1183

• P Falkowski, P Mrozek, Z Lukaszewsk, L Oldak, E Gorodkiewi An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi-A Comparison of Analytical Properties of Silver-Gold and Pure Gold Chips Biosensors, 2021-08-27;11(9):. 2021-08-27 [PMID: 34562888] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: CTSS
• Uniprot:
  • P25774()