Cathepsin L Antibody [Unconjugated]

Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cathepsin L was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse liver tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin L at approximately 51 and 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Cathepsin L was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Acute, first window RIC activates autophagy signaling via p-AMPK upregulation and concomitant downregulation of mTOR.(A) Western blots for autophagy related signaling proteins. (B) Quantification of the protein fold change in 1W RIC compared to 1W controls. Values are means ± S.E.M. n = 6–8 per group. An (*) denotes a statistically significant difference (P<0.05) compared to control. (P-: phospho-). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25347774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chronic, third window RIC increases the expression of autophagosome proteins, LC3I/II and Atg5.(A) Western blots for autophagy related signaling proteins. (B) Quantification of the protein fold change in 3W RIC compared to 3W controls. Values are means ± S.E.M. n = 6–8 per group. An (*) denotes a statistically significant difference (P<0.05) compared to control. (P-: phospho-). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25347774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of CTSV induces cellular senescence. (A) HUVECs were treated with si-negative control (NC), si-CTSV, si-ALDH1A2 or si-CTSV + si-ALDH1A2 for 24 h and simulated with 100 nM doxorubicin (DOX) for 24 h after incubated in complete growth medium for 24 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, si-CTSV and si-ALDH1A2 (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53 and P16 in HUVECs treated with DOX and siRNAs (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6 and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX and siRNAs (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ctsl expression is decreased in senescent aorta. (A) Representative images of hematoxylin-eosin staining in the thoracic aortas of aged (18-month-old) and young (8-week-old) mice (scale bar = 200 μm in top and 50 μm in bottom, respectively). (B) Representative images of Masson’s trichrome stain in the thoracic aortas (blue-gray staining for the collagens, scale bar = 200 μm in top and 50 μm in bottom, respectively). (C) Quantification of media thickness (MT), the ratio of media thickness to lumen diameter (MT/LD), media area (MA), and the ratio of collagen to media area (collagen/MA) using hematoxylin-eosin staining and Masson’s trichrome stain (n = 4). (D). Immunohistochemistry of Ctsl in the thoracic aortas of aged and young mice (brown staining for Ctsl, scale bar = 50 μm, n = 4). (E) Western Blot of Ctsl and p21 in young and senescent aortas (n = 3–4). Data were presented as mean ± SEM. T-test was used. * p < 0.05 vs. young, ** p < 0.01 vs. young, *** p < 0.001 vs. young. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

CTSV attenuates DOX-induced cellular senescence. (A). HUVECs were treated with ad-negative control (NC) or ad-CTSV (MOI = 10) for 8 h and simulated with 100 nM doxorubicin (DOX) and 1 μM RA for 24 h after incubated in complete growth medium for 40 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, RA and ad-CTSV (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53, and P16 in HUVECs treated with DOX, RA and ad-CTSV (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6, and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX, RA, and ad-CTSV (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ctsl expression is decreased in senescent aorta. (A) Representative images of hematoxylin-eosin staining in the thoracic aortas of aged (18-month-old) and young (8-week-old) mice (scale bar = 200 μm in top and 50 μm in bottom, respectively). (B) Representative images of Masson’s trichrome stain in the thoracic aortas (blue-gray staining for the collagens, scale bar = 200 μm in top and 50 μm in bottom, respectively). (C) Quantification of media thickness (MT), the ratio of media thickness to lumen diameter (MT/LD), media area (MA), and the ratio of collagen to media area (collagen/MA) using hematoxylin-eosin staining and Masson’s trichrome stain (n = 4). (D). Immunohistochemistry of Ctsl in the thoracic aortas of aged and young mice (brown staining for Ctsl, scale bar = 50 μm, n = 4). (E) Western Blot of Ctsl and p21 in young and senescent aortas (n = 3–4). Data were presented as mean ± SEM. T-test was used. * p < 0.05 vs. young, ** p < 0.01 vs. young, *** p < 0.001 vs. young. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of CTSV induces cellular senescence. (A) HUVECs were treated with si-negative control (NC), si-CTSV, si-ALDH1A2 or si-CTSV + si-ALDH1A2 for 24 h and simulated with 100 nM doxorubicin (DOX) for 24 h after incubated in complete growth medium for 24 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, si-CTSV and si-ALDH1A2 (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53 and P16 in HUVECs treated with DOX and siRNAs (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6 and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX and siRNAs (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

CTSV attenuates DOX-induced cellular senescence. (A). HUVECs were treated with ad-negative control (NC) or ad-CTSV (MOI = 10) for 8 h and simulated with 100 nM doxorubicin (DOX) and 1 μM RA for 24 h after incubated in complete growth medium for 40 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, RA and ad-CTSV (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53, and P16 in HUVECs treated with DOX, RA and ad-CTSV (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6, and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX, RA, and ad-CTSV (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin L Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin L
  Thr18-Asn334
  Accession # P06797
• Specificity: Detects mouse Cathepsin L in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ctsl
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody [Unconjugated]

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha ₁-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). In Cathepsin L-deficient mice, it appears to play a critical role in cardiac morphology and function, epidermal homeostasis, regulation of the hair cycle, and MHC class II-mediated antigen presentation in cortical epithelial cells of the thymus (4, 5). Mouse Cathepsin L is synthesized as a 334 amino acid precursor with a signal peptide (residues 1-17), a pro region (residues 18-113), and a mature chain (residues 114-334).

1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
4. Stypmann, J. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 6234.
5. Reinheckel, T. et al. (2001) Biol. Chem. 382:735.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (AF1515)(54)

Publications using AF1515

• Samanta, S;Nandi, A;Datta, R;Dolai, S; Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages Journal of cell science 2025-08-01 [PMID: 40641446] (Immunocytochemistry, Mouse)
• Demcsák, A;Tran, T;Sahin-Tóth, M;Geisz-Fremy, A; Strain-specific differences in cerulein-induced acute and recurrent acute murine pancreatitis Scientific reports 2025-05-08 [PMID: 40341748] (Western Blot, Mouse)
• Katagiri, D;Nagasaka, S;Takahashi, K;Wang, S;Pozzi, A;Zent, R;Shimizu, A;Zhang, MZ;Göthert, JR;van Kuppevelt, TH;Harris, RC;Takahashi, T; Endothelial eNOS deficiency causes podocyte injury through NFAT2 and heparanase in diabetic mice Scientific reports 2024-11-25 [PMID: 39587144] (Immunohistochemistry, Transgenic Mouse)
• Do?ru, AG;Rehders, M;Brix, K; Investigations on Primary Cilia of Nthy-ori 3-1 Cells upon Cysteine Cathepsin Inhibition or Thyrotropin Stimulation International journal of molecular sciences 2023-05-26 [PMID: 37298246] (Western Blot, Human)
• Chan Li, Zhaoya Liu, Mengshi Chen, Liyang Zhang, Ruizheng Shi, Hua Zhong Critical Role of Cathepsin L/V in Regulating Endothelial Cell Senescence Biology (Basel) 12/26/2022 [PMID: 36671735]
• H Zhang, Y Chen, F Li, C Wu, W Cai, H Ye, H Su, M He, L Yang, X Wang, K Zhou, W Ni Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization Journal of Neuroinflammation, 2023-01-07;20(1):6. 2023-01-07 [PMID: 36609266] (IHC, Western Blot, Mouse)
• M Polesel, M Kaminska, D Haenni, M Bugarski, C Schuh, N Jankovic, A Kaech, JM Mateos, M Berquez, AM Hall Spatiotemporal organisation of protein processing in the kidney Nature Communications, 2022-09-29;13(1):5732. 2022-09-29 [PMID: 36175561] (IHC, Mouse)
• E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022-08-17;13(1):4848. 2022-08-17 [PMID: 35977928] (Western Blot, Mouse)
• Z Cui, C Zeng, F Huang, F Yuan, J Yan, Y Zhao, Y Zhou, W Hankey, VX Jin, J Huang, HF Staats, JI Everitt, GD Sempowski, H Wang, Y Dong, SL Liu, Q Wang Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection Oncogene, 2022-07-25;0(0):. 2022-07-25 [PMID: 35879545] (IHC, Western Blot, Mouse)
• T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022-03-14;10(1):33. 2022-03-14 [PMID: 35287730] (Western Blot, Mouse)
• TT Dang, SH Back Translation Inhibitors Activate Autophagy Master Regulators TFEB and TFE3 International Journal of Molecular Sciences, 2021-11-08;22(21):. 2021-11-08 [PMID: 34769510] (Western Blots, Mouse)
• Suchitra Kamle, Bing Ma, Chuan Hua He, Bedia Akosman, Yang Zhou, Chang-Min Lee et al. Chitinase 3-like-1 is a therapeutic target that mediates the effects of aging in COVID-19 JCI Insight 11/8/2021 [PMID: 34747367]
• Michel Nofal, Tim Wang, Lifeng Yang, Connor S.R. Jankowski, Sophia Hsin-Jung Hsin-Jung Li, Seunghun Han et al. GCN2 adapts protein synthesis to scavenging-dependent growth Cell Systems 2/1/2022 [PMID: 34706266]
• Michel Nofal, Tim Wang, Lifeng Yang, Connor S.R. Jankowski, Sophia Hsin-Jung Hsin-Jung Li, Seunghun Han et al. GCN2 adapts protein synthesis to scavenging-dependent growth Cell Systems 2022-02-01 [PMID: 34706266] (Western Blot, Mouse)
• Du H, Wong MY, Zhang T et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses Life science alliance 7/1/2021 [PMID: 34103390]
• L Liu, B Doray Elevated mRNA expression and defective processing of cathepsin D in HeLa cells lacking the mannose 6-phosphate pathway FEBS Open Bio, 2021-05-05;0(0):. 5/5/2021 [PMID: 33932147]
• M Shibamura-, K Yuki, L Hou Cathepsin L regulates pathogenicCD4 T cells in experimental autoimmune encephalomyelitis International immunopharmacology, 2021-02-01;93(0):107425. 2021-02-01 [PMID: 33540247] (Western Blot, Mouse)
• S Kamle, B Ma, CH He, B Akosman, Y Zhou, CM Lee, WS El-Deiry, K Huntington, O Liang, J Machan, MJ Kang, HJ Shin, E Mizoguchi, CG Lee, JA Elias Chitinase 3-like-1 is a Therapeutic Target That Mediates the Effects of Aging in COVID-19 bioRxiv : the preprint server for biology, 2021-02-16;0(0):. 2021-02-16 [PMID: 33442679] (IHC, Western Blot, Mouse)
• S Ketterer, J Mitschke, A Ketscher, M Schlimpert, W Reichardt, N Baeuerle, ME Hess, P Metzger, M Boerries, C Peters, B Kammerer, T Brummer, F Steinberg, T Reinheckel Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling Nat Commun, 2020-10-12;11(1):5133. 2020-10-12 [PMID: 33046706] (Western Blot, Mouse)
• LM Westermann, L Fleischhau, J Vogel, Z Jenei-Lanz, N Floriano L, L Schau, F Morellini, A Baranowsky, TA Yorgan, G Di Lorenzo, M Schweizer, B de Souza P, NR Guarany, F Sperb-Ludw, F Visioli, T Oliveira S, J Soul, G Hendrickx, JS Wiegert, IVD Schwartz, H Clausen-Sc, F Zaucke, T Schinke, S Pohl, T Danyukova Imbalanced cellular metabolism compromises cartilage homeostasis and joint function in a mouse model of mucolipidosis type III gamma Dis Model Mech, 2020-11-18;0(0):. 2020-11-18 [PMID: 33023972] (Western Blot, Mouse)
• Georg Werner, Markus Damme, Martin Schludi, Johannes Gnörich, Karin Wind, Katrin Fellerer et al. Loss of TMEM 106B potentiates lysosomal and FTLD ‐like pathology in progranulin‐deficient mice EMBO reports 10/5/2020 [PMID: 32929860]
• Feng T, Mai S, Roscoe JM et al. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice EMBO Rep. 8/10/2020 [PMID: 32852886]
• MA Parigiani, A Ketscher, S Timme, P Bronsert, M Schlimpert, B Kammerer, A Jacquel, P Chaintreui, T Reinheckel Conditional Gene Targeting Reveals Cell Type-Specific Roles of the Lysosomal Protease Cathepsin L in Mammary Tumor Progression Cancers (Basel), 2020-07-22;12(8):. 2020-07-22 [PMID: 32707827] (Western Blot, Mouse)
• Panos G. Ziros, Cédric O. Renaud, Dionysios V. Chartoumpekis, Massimo Bongiovanni, Ioannis G. Habeos, Xiao-Hui Liao et al. Mice Hypomorphic for Keap1, a Negative Regulator of the Nrf2 Antioxidant Response, Show Age-Dependent Diffuse Goiter with Elevated Thyrotropin Levels Thyroid 2021-01-01 [PMID: 32689903] (Western Blot, Transgenic Mouse)
• Panos G. Ziros, Cédric O. Renaud, Dionysios V. Chartoumpekis, Massimo Bongiovanni, Ioannis G. Habeos, Xiao-Hui Liao et al. Mice Hypomorphic for Keap1, a Negative Regulator of the Nrf2 Antioxidant Response, Show Age-Dependent Diffuse Goiter with Elevated Thyrotropin Levels Thyroid 1/1/2021 [PMID: 32689903]
• Limor Goren, George Zhang, Susmita Kaushik, Paul A. S. Breslin, Yi-Chieh Nancy Du, David A. Foster (-)-Oleocanthal and (-)-oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells PLOS ONE 8/14/2019 [PMID: 31412041]
• Bullard WL, Kara M, Gay LA et al. Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery PLoS Pathog. 8/1/2019 [PMID: 31393953]
• Y Li, JW Jones, H M C Choi, C Sarkar, MA Kane, EY Koh, MM Lipinski, J Wu cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury Cell Death Dis, 2019-07-11;10(7):531. 7/11/2019 [PMID: 31296844]
• C Sarkar, JW Jones, N Hegdekar, JA Thayer, A Kumar, AI Faden, MA Kane, MM Lipinski PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma Autophagy, 2019-06-25;0(0):1-20. 2019-06-25 [PMID: 31238788] (ICC, IHC, Mouse)
• Weiss Sadan, T;Itzhak, G;Kaschani, F;Yu, Z;Mahameed, M;Anaki, A;Ben-Nun, Y;Merquiol, E;Tirosh, B;Kessler, B;Kaiser, M;Blum, G; Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation Mol. Cell Proteomics 4/22/2019 [PMID: 31010818]
• AN Parks, J Nahata, NE Edouard, JS Temenoff, MO Platt Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis Sci Rep, 2019-04-01;9(1):5399. 2019-04-01 [PMID: 30931961] (Western Blot, Human)
• JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice Mol Neurodegener, 2018-09-04;13(1):48. 2018-09-04 [PMID: 30180904] (Western Blot, Mouse)
• B Lloyd-Lewi, CC Krueger, TJ Sargeant, ME D'Angelo, MJ Deery, R Feret, JA Howard, KS Lilley, CJ Watson Stat3 mediated alterations in lysosomal membrane protein composition J. Biol. Chem., 2018-01-17;0(0):. 2018-01-17 [PMID: 29343516] (Western Blot, Mouse)
• Zoe A. Klein, Hideyuki Takahashi, Mengxiao Ma, Massimiliano Stagi, Melissa Zhou, TuKiet T. Lam et al. Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice Neuron 7/19/2017 [PMID: 28728022]
• C Cvetkovic, MC Ferrall-Fa, E Ko, L Grant, H Kong, MO Platt, R Bashir Investigating the Life Expectancy and Proteolytic Degradation of Engineered Skeletal Muscle Biological Machines Sci Rep, 2017-06-19;7(1):3775. 2017-06-19 [PMID: 28630410] (Western Blot, Mouse)
• I Khamaysi, P Singh, S Nasser, H Awad, Y Chowers, E Sabo, E Hammond, I Gralnek, I Minkov, A Noseda, N Ilan, I Vlodavsky, Z Abassi The Role of Heparanase in the Pathogenesis of Acute Pancreatitis: A Potential Therapeutic Target Sci Rep, 2017-04-06;7(1):715. 2017-04-06 [PMID: 28386074] (IHC, Mouse)
• MG Boels, EE van Faasse, MC Avramut, J van der Vl, BM van den Be, TJ Rabelink Direct Observation of Enhanced Nitric Oxide in a Murine Model of Diabetic Nephropathy PLoS ONE, 2017-01-19;12(1):e0170065. 2017-01-19 [PMID: 28103268] (IHC-P, Mouse)
• CCT complex restricts neuropathogenic protein aggregation via autophagy Nat Commun, 2016-12-08;7(0):13821. 12/8/2016 [PMID: 27929117]
• Lifei Hou, Jessica Cooley, Richard Swanson, Poh Chee Ong, Robert N. Pike, Matthew Bogyo et al. The protease cathepsin L regulates Th17 cell differentiation Journal of Autoimmunity 12/1/2015 [PMID: 26343333]
• Lifei Hou, Jessica Cooley, Richard Swanson, Poh Chee Ong, Robert N. Pike, Matthew Bogyo et al. The protease cathepsin L regulates Th17 cell differentiation Journal of Autoimmunity 2015-12-01 [PMID: 26343333] (Western Blot, Mouse)
• Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015-04-30;10(4):e0120348. 2015-04-30 [PMID: 25927437] (IHC, Mouse)
• Kissing S, Hermsen C, Repnik U, Nesset C, von Bargen K, Griffiths G, Ichihara A, Lee B, Schwake M, De Brabander J, Haas A, Saftig P Vacuolar ATPase in phagosome-lysosome fusion. J Biol Chem, 2015-04-22;290(22):14166-80. 2015-04-22 [PMID: 25903133] (Western Blot, Mouse)
• Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015-02-15;14(0):39. 2015-02-15 [PMID: 25744631] (Western Blot, Mouse)
• Rohailla S, Clarizia N, Sourour M et al. Acute, Delayed and Chronic Remote Ischemic Conditioning Is Associated with Downregulation of mTOR and Enhanced Autophagy Signaling. PLoS OnE. 10/28/2014 [PMID: 25347774]
• Timothy J. Sargeant, Bethan Lloyd-Lewis, Henrike K. Resemann, Antonio Ramos-Montoya, Jeremy Skepper, Christine J. Watson Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization Nature Cell Biology 11/1/2014 [PMID: 25283994]
• Timothy J. Sargeant, Bethan Lloyd-Lewis, Henrike K. Resemann, Antonio Ramos-Montoya, Jeremy Skepper, Christine J. Watson Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization Nature Cell Biology 2014-11-01 [PMID: 25283994] (Western Blot, Mouse)
• Martina Tholen, Larissa E. Hillebrand, Stefan Tholen, Oliver Sedelmeier, Sebastian J. Arnold, Thomas Reinheckel Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo Nature Communications 9/15/2014 [PMID: 25222295]
• Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014-06-06;21(9):1442-50. 2014-06-06 [PMID: 24902901] (Western Blot, Mouse)
• Ohkouchi , Susumu, Shibata , Masahiro, Sasaki , Mitsuho, Koike , Masato, Safig , Paul, Peters , Christop, Nagata , Shigekaz, Uchiyama , Yasuo Biogenesis and proteolytic processing of lysosomal DNase II. PLoS ONE, 2013-03-13;8(3):e59148. 2013-03-13 [PMID: 23516607] (IHC, Mouse)
• Jennifer Hughes, Susana Frago, Claudia Bühnemann, Emma J. Carter, A. Bassim Hassan Maternal Transmission of a Humanised Igf2r Allele Results in an Igf2 Dependent Hypomorphic and Non-Viable Growth Phenotype PLoS ONE 2/28/2013 [PMID: 23468951]
• Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011-12-01;25(23):2465-79. 2011-12-01 [PMID: 22156207] (Western Blot, Mouse)
• Margot G. Paulick, Matthew Bogyo Development of Activity-Based Probes for Cathepsin X ACS Chemical Biology 6/17/2011 [PMID: 21322635]
• Funkelstein L, Toneff T, Mosier C, Hwang SR, Beuschlein F, Lichtenauer UD, Reinheckel T, Peters C, Hook V Major Role of Cathepsin L for Producing the Peptide Hormones ACTH, beta-Endorphin, and alpha-MSH, Illustrated by Protease Gene Knockout and Expression. J. Biol. Chem., 2008-10-10;283(51):35652-9. 2008-10-10 [PMID: 18849346] (IHC-Fr, Mouse)
• Baek D, Villen J, Shin C, Camargo FD, Gygi SP, Bartel DP The impact of microRNAs on protein output. Nature, 2008-07-30;455(7209):64-71. 2008-07-30 [PMID: 18668037] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ctsl
• Uniprot:
  • P06797()