Cathepsin L Antibody [Unconjugated]

Cathepsin L was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cathepsin L was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse liver tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin L at approximately 51 and 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin L Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin L
  Thr18-Asn334
  Accession # P06797
• Specificity: Detects mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Cathepsin L is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ctsl

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody [Unconjugated]

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha ₁-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). In Cathepsin L-deficient mice, it appears to play a critical role in cardiac morphology and function, epidermal homeostasis, regulation of the hair cycle, and MHC class II-mediated antigen presentation in cortical epithelial cells of the thymus (4, 5). Mouse Cathepsin L is synthesized as a 334 amino acid precursor with a signal peptide (residues 1-17), a pro region (residues 18-113), and a mature chain (residues 114-334).

1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
4. Stypmann, J. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 6234.
5. Reinheckel, T. et al. (2001) Biol. Chem. 382:735.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (AF1515)(14)

Publications using AF1515

• AN Parks, J Nahata, NE Edouard, JS Temenoff, MO Platt Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis Sci Rep, 2019;9(1):5399. 2019 [PMID: 30931961] (Western Blot, Human)
• JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice Mol Neurodegener, 2018;13(1):48. 2018 [PMID: 30180904] (Western Blot, Mouse)
• B Lloyd-Lewi, CC Krueger, TJ Sargeant, ME D'Angelo, MJ Deery, R Feret, JA Howard, KS Lilley, CJ Watson Stat3 mediated alterations in lysosomal membrane protein composition J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29343516] (Western Blot, Mouse)
• C Cvetkovic, MC Ferrall-Fa, E Ko, L Grant, H Kong, MO Platt, R Bashir Investigating the Life Expectancy and Proteolytic Degradation of Engineered Skeletal Muscle Biological Machines Sci Rep, 2017;7(1):3775. 2017 [PMID: 28630410] (Western Blot, Mouse)
• I Khamaysi, P Singh, S Nasser, H Awad, Y Chowers, E Sabo, E Hammond, I Gralnek, I Minkov, A Noseda, N Ilan, I Vlodavsky, Z Abassi The Role of Heparanase in the Pathogenesis of Acute Pancreatitis: A Potential Therapeutic Target Sci Rep, 2017;7(1):715. 2017 [PMID: 28386074] (IHC, Mouse)
• MG Boels, EE van Faasse, MC Avramut, J van der Vl, BM van den Be, TJ Rabelink Direct Observation of Enhanced Nitric Oxide in a Murine Model of Diabetic Nephropathy PLoS ONE, 2017;12(1):e0170065. 2017 [PMID: 28103268] (IHC-P, Mouse)
• Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015;10(4):e0120348. 2015 [PMID: 25927437] (IHC, Mouse)
• Kissing S, Hermsen C, Repnik U, Nesset C, von Bargen K, Griffiths G, Ichihara A, Lee B, Schwake M, De Brabander J, Haas A, Saftig P Vacuolar ATPase in phagosome-lysosome fusion. J Biol Chem, 2015;290(22):14166-80. 2015 [PMID: 25903133] (Western Blot, Mouse)
• Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015;14(0):39. 2015 [PMID: 25744631] (Western Blot, Mouse)
• Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014;21(9):1442-50. 2014 [PMID: 24902901] (Western Blot, Mouse)
• Ohkouchi, Susumu, Shibata, Masahiro, Sasaki, Mitsuho, Koike, Masato, Safig, Paul, Peters, Christop, Nagata, Shigekaz, Uchiyama, Yasuo Biogenesis and proteolytic processing of lysosomal DNase II. PLoS ONE, 2013;8(3):e59148. 2013 [PMID: 23516607] (IHC, Mouse)
• Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011;25(23):2465-79. 2011 [PMID: 22156207] (Western Blot, Mouse)
• Funkelstein L, Toneff T, Mosier C, Hwang SR, Beuschlein F, Lichtenauer UD, Reinheckel T, Peters C, Hook V Major Role of Cathepsin L for Producing the Peptide Hormones ACTH, beta-Endorphin, and alpha-MSH, Illustrated by Protease Gene Knockout and Expression. J. Biol. Chem., 2008;283(51):35652-9. 2008 [PMID: 18849346] (IHC-Fr, Mouse)
• Baek D, Villen J, Shin C, Camargo FD, Gygi SP, Bartel DP The impact of microRNAs on protein output. Nature, 2008;455(7209):64-71. 2008 [PMID: 18668037] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ctsl
• Uniprot:
  • P06797()