Cathepsin L Antibody (193702)

• Reactivity: I
• Applications: WB, IP
• Clone: 193702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin L Antibody (193702) Summary

• Immunogen: S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L
• Specificity: Detects pro and mature forms of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Cathepsin L is observed and no cross-reactivity with recombinant mouse Cathepsin L is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSL1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody (193702)

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (MAB22591)(6)

Publications using MAB22591

• AA Mondragon, A Yalonetska, AJ Ortega, Y Zhang, O Naranjo, J Elguero, WS Chung, K McCall Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death Cell Rep, 2019;27(1):11-19.e3. 2019 [PMID: 30943394] (IHC, Insect - Drosophila)
• M ?entürk, G Lin, Z Zuo, D Mao, E Watson, AG Mikos, HJ Bellen Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification Nat. Cell Biol., 2019;21(3):384-396. 2019 [PMID: 30804504] (Western Blot, Insect)
• D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway Autophagy, 2019;0(0):. 2019 [PMID: 30741620] (IHC, Human)
• C Li, JM Brazill, S Liu, C Bello, Y Zhu, M Morimoto, L Cascio, R Pauly, Z Diaz-Perez, MCV Malicdan, H Wang, L Boccuto, CE Schwartz, WA Gahl, CF Boerkoel, RG Zhai Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome Nat Commun, 2017;8(1):1257. 2017 [PMID: 29097652] (Western Blot, Drosophila)
• Luan S, Ilvarsonn A, Eissenberg J The unique GGA clathrin adaptor of Drosophila melanogaster is not essential. PLoS ONE, 2012;7(9):e45163. 2012 [PMID: 23028818] (Western Blot, Drosophila)
• Johnson GD, Jiang W Characterization of cathepsin L secreted by Sf21 insect cells. Arch. Biochem. Biophys., 2005;444(1):7-14. 2005 [PMID: 16274658] (Immunoprecipitation, Insect)

Bioinformatics

• Gene Symbol: CTSL1